Author | Campos, Fernanda Magalhães Freire | |
Author | Repoles, Laura Cotta | |
Author | Araújo, Fernanda Fortes de | |
Author | Pascoal, Vanessa Peruhype Magalhães | |
Author | Xavier, Marcelo Antônio Pascoal | |
Author | Sabino, Ester Cerdeira | |
Author | Proietti, Anna Bárbara de Freitas Carneiro | |
Author | Andrade, Mariléia Chaves de | |
Author | Carvalho, Andréa Teixeira de | |
Author | Martins Filho, Olindo Assis | |
Author | Gontijo, Célia Maria Ferreira | |
Access date | 2018-09-17T13:30:54Z | |
Available date | 2018-09-17T13:30:54Z | |
Document date | 2018 | |
Citation | CAMPOS, Fernanda Magalhães Freire et al. Usefulness of FC-TRIPLEX Chagas/Leish IgG1 as confirmatory assay for non-negative results in blood bank screening of Chagas disease. J Immunol Methods., v. 455, p. 34-40, 2018 | pt_BR |
ISSN | 0022-1759 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28824 | |
Language | eng | pt_BR |
Publisher | Elsevier Inc | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | banco de sangue | pt_BR |
Subject in Portuguese | Doença de chagas | pt_BR |
Subject in Portuguese | Ensaio confirmatório | pt_BR |
Subject in Portuguese | FC-TRIPLEX Chagas/Leish IgG1 | pt_BR |
Subject in Portuguese | citometria de fluxo | pt_BR |
Subject in Portuguese | sorologia | pt_BR |
Title | Usefulness of FC-TRIPLEX Chagas/Leish IgG1 as confirmatory assay for non-negative results in blood bank screening of Chagas disease | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jim.2018.01.006 | |
Abstract | A relevant issue in Chagas disease serological diagnosis regards the requirement of using several confirmatory methods to elucidate the status of non-negative results from blood bank screening. The development of a single reliable method may potentially contribute to distinguish true and false positive results. Our aim was to evaluate the performance of the multiplexed flow-cytometry anti-T. cruzi/Leishmania IgG1 serology/(FC-TRIPLEX Chagas/Leish IgG1) with three conventional confirmatory criteria (ELISA-EIA, Immunofluorescence assay-IIF and EIA/IIF consensus criterion) to define the final status of samples with actual/previous non-negative results during anti-T. cruzi ELISA-screening in blood banks. Apart from inconclusive results, the FC-TRIPLEX presented a weak agreement index with EIA, while a strong agreement was observed when either IIF or EIA/IIF consensus criteria were applied. Discriminant analysis and Spearman's correlation further corroborates the agreement scores. ROC curve analysis showed that FC-TRIPLEX performance indexes were higher when IIF and EIA/IIF consensus were used as a confirmatory criterion. Logistic regression analysis further demonstrated that the probability of FC-TRIPLEX to yield positive results was higher for inconclusive results from IIF and EIA/IIF consensus. Machine learning tools illustrated the high level of categorical agreement between FC-TRIPLEX versus IIF or EIA/IIF consensus. Together, these findings demonstrated the usefulness of FC-TRIPLEX as a tool to elucidate the status of non-negative results in blood bank screening of Chagas disease. | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pós graduação em Sanidade e Produção Animal nos Trópicos, Uberaba, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Pró-Sangue. São Paulo, SP, Brazil/Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brazil | pt_BR |
Affilliation | Fundação Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Montes Claros, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Blood bank | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Confirmatory assay | pt_BR |
Subject | FC-TRIPLEX Chagas/Leish IgG1 | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | Serology | pt_BR |
Embargo date | 2024-01-01 | |