Author | Jesus, Jose Batista de | |
Author | Cuervo, Patrícia | |
Author | Britto, Constança | |
Author | Sabóia-Vahia, Leonardo | |
Author | Silva Filho, Fernando Costa e | |
Author | Veloso, Andre Borges | |
Author | Petrópolis, Débora Barreiros | |
Author | Cupolillo, Elisa | |
Author | Domont, Gilberto Barbosa | |
Access date | 2018-08-23T15:42:08Z | |
Available date | 2018-08-23T15:42:08Z | |
Document date | 2009 | |
Citation | JESUS, Jose Batista de; et al. Cysteine Peptidase Expression in Trichomonas vaginalis Isolates Displaying High- and Low-Virulence Phenotypes. Journal of Proteome Research, v.8, p.1555-1564, 2009. | pt_BR |
ISSN | 1535-3893 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28332 | |
Language | eng | pt_BR |
Publisher | American Chemical Society | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trichomonas vaginalis | pt_BR |
Subject in Portuguese | protozoário parasita | pt_BR |
Subject in Portuguese | eletroforese bidimensional | pt_BR |
Subject in Portuguese | espectrometria de massa | pt_BR |
Subject in Portuguese | citotoxicidade parasitária | pt_BR |
Subject in Portuguese | Cisteína | pt_BR |
Title | Cysteine peptidase expression in Trichomonas vaginalis isolates displaying high- and low-virulence phenotypes | pt_BR |
Type | Article | pt_BR |
DOI | 10.1021/pr8009066 | |
Abstract | In the present study, we identified and characterized the cysteine peptidase (CP) profiles of Trichomonas vaginalis isolates exhibiting high- and low-virulence phenotypes using a combination of two-dimensional SDS-PAGE (2DE), tandem mass spectrometry (MS/MS), and data mining. Seven of the eight CPs identified belong to Clan CA, family C1, cathepsin L-like CP, and one belongs to Clan CD, family C13, asparaginyl endopeptidase-like CP. Quantitative and qualitative differences in CP expression were detected between the isolates. BLAST analysis followed by CLUSTAL alignment of amino acid sequences of differentially expressed CPs showed identity or high homology to previously described CP cDNA clones CP1, CP3, CP4, and to a secreted CP fraction of 30 kDa involved in apoptosis of vaginal epithelial cells. One- and two-dimensional-substrate gel analyses revealed the differential CP profiles between the isolates, indicating that the combination of zymography with 2DE and MS/MS might be a powerful experimental approach to map and identify active peptidases in T. vaginalis. Toxicity exerted upon HeLa cells by high- and low-virulence isolates was 98.3% and 31%, respectively. Pretreatment of parasites with specific Clan CA papain-like CP inhibitor l-3-carboxy-2,3-trans-epoxypropionyl-leucylamido(4-guanidino)butane (E-64) drastically reduced the cytotoxic effect to 21.7% and 0.8%, respectively, suggesting that T. vaginalis papain-like CPs are the main factors involved in the cellular damage. | pt_BR |
Affilliation | Universidade Federal de São João del Rei. Departamento de Ciências Naturais. São Joâo del rei, MG, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Bioengenharia de Sistemas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de São João del Rei. São João del Rei, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Bioengenharia de Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trichomonas vaginalis | pt_BR |
Subject | parasitic protozoan | pt_BR |
Subject | cysteine peptidase | pt_BR |
Subject | metallopeptidase | pt_BR |
Subject | twodimensional electrophoresis | pt_BR |
Subject | mass spectrometry | pt_BR |
Subject | parasite cytotoxicity | pt_BR |
e-ISSN | 1535-3907 | |
Embargo date | 2030-01-01 | |