Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/2800
Title: Physalins B, F and G, seco-steroids purified from Physalis angulata L., inhibit lymphocyte function and allogeneic transplant rejection
Authors: Soares, Milena Botelho Pereira
Brustolim, Daniele
Santos, Luciane Amorim
Bellintani, Moema Cortizo
Paiva, F. P
Ribeiro, Yvone M
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Physalis angulata is a solanaceae widely used in folk medicine in various tropical countries in the world. We have previously described that seco-steroids (physalins) purified from P. angulata are potent inhibitors of macrophage activation, blocking the production of pro-inflammatory cytokines and LPS-induced lethality. Herein we investigated the immunomodulatory activities of these substances in lymphocyte proliferation and cytokine production and in transplantation. The addition of physalins B, F or G to concanavalin A-activated splenocyte cultures induced a concentration-dependent inhibition of proliferation. Physalin B also inhibited IL-2 production by Con A-activated spleen cells. The addition of 2 Ag/ml physalin B to mixed lymphocyte reaction (MLR) caused a 100% inhibition of proliferation. More importantly, treatment of mice with physalin B, F or G prevented the rejection of allogeneic heterotopic heart transplant. Our results demonstrate the suppressive activity of physalins B, F and G in lymphocyte function and indicate the potential use of physalins as immunosuppressive agents for treatments of pathologies in which inhibition of immune responses is desired.
Keywords: Physalin
Immunosuppression
Lymphoproliferation
Transplantation
keywords: Imunossupressão
Linfoproliferação
Transplantação
DeCS: Animais
Proliferação de Células
Concanavalina A
Feminino
Rejeição de Enxerto
Transplante de Coração
Imunossupressores
Interleucina-2
Linfócitos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C3H
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos CBA
Mifepristona
Physalis
Secoesteróides
Baço
Issue Date: 2006
Publisher: Elsevier
Citation: SOARES, M. B. P. et al. Physalins B, F and G, seco-steroids purified from Physalis angulata L., inhibit lymphocyte function and allogeneic transplant rejection. International Immunopharmacology, v.6, n.3, p.408-414, 2006.
Description: Os autores gostariam de agradecer aos Drs. Augusto C. A. Mota e Washington L. C. Santos que gentilmente ajudaram com as análises estatísticas.
DOI: 10.1016/j.intimp.2005.09.007
ISSN: 1567-5769
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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