Author | Miranda, Aline Cristina Andrade Mota | |
Author | Oliveira, Tulio de | |
Author | Moreau, Domingos Ramon | |
Author | Bomfim, Catarina | |
Author | Castro Filho, Bernardo Galvão | |
Author | Alcantara, Luiz Carlos Júnior | |
Access date | 2011-08-16T13:03:01Z | |
Available date | 2011-08-16T13:03:01Z | |
Document date | 2007 | |
Citation | MOTA-MIRANDA, A. C. et al. Mapping the molecular characteristics of Brazilian human T-cell lymphotropic virus type 1 Env (gp46) and Pol amino acid sequences for vaccine design. Memórias do Instituto Oswaldo Cruz, v.102, n.6, p.741-749, set. 2007. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/2797 | |
Description | Agradecimentos a Elisabeth Deliège pela assistência técnica. | pt_BR |
Sponsorship | Apoio financeiro: LSPA / CPqGM / Fiocruz, Fapesb (concessão 303/03), Ministério da Saúde do Brasil(subvenções 306/04, 307/04). | pt_BR |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | |
Subject in Portuguese | Virus Linfotrópico de Células T Humanas Tipo 1 | pt_BR |
Subject in Portuguese | Sequencia Env | pt_BR |
Subject in Portuguese | Sequencia Pol | pt_BR |
Subject in Portuguese | Epitopos | pt_BR |
Title | Mapping the molecular characteristics of Brazilian human T-cell lymphotropic virus type 1 Env (gp46) and Pol amino acid sequences for vaccine design | pt_BR |
Type | Article | pt_BR |
Abstract | This study was carried out to evaluate the molecular pattern of all available Brazilian human T-cell lymphotropic virus type 1 Env (n = 15) and Pol (n = 43) nucleotide sequences via epitope prediction, physicochemical
analysis, and protein potential sites identification, giving support to the Brazilian AIDS vaccine program. In 12 previously described peptides of the Env sequences we found 12 epitopes, while in 4 peptides of the Pol sequences we found 4 epitopes. The total variation on the amino acid composition was 9 and 17% for human leukocyte antigen (HLA) class I and class II Env epitopes, respectively. After analyzing the Pol sequences, results revealed a total amino acid variation of 0.75% for HLA-I and HLA-II epitopes. In 5 of the 12 Env epitopes
the physico-chemical analysis demonstrated that the mutations magnified the antigenicity profile. The potential protein domain analysis of Env sequences showed the loss of a CK-2 phosphorylation site caused by D197N
mutation in one epitope, and a N-glycosylation site caused by S246Y and V247I mutations in another epitope. Besides, the analysis of selection pressure have found 8 positive selected sites (w = 9.59) using the codon-based substitution models and maximum-likelihood methods. These studies underscore the importance of this Env region for the virus fitness, for the host immune response and, therefore, for the development of vaccine candidates. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Bahiana para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | South African National Bioinformatics Institute. MRC Pathogen Bioinformatics Unit. UWC, Cape Town, South Africa / Oxford University. Zoology Department. Oxford, United Kingdom | pt_BR |
Affilliation | South African National Bioinformatics Institute. MRC Pathogen Bioinformatics Unit. UWC, Cape Town, South Africa / Oxford University. Zoology Department. Oxford, United Kingdom | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública / Fundação para o Desenvolvimento da Ciência. Salvador, BA, Brasil | pt_BR |
Subject | Human T-cell lymphotropic virus type 1 | pt_BR |
Subject | Env | pt_BR |
Subject | Pol | pt_BR |
Subject | Epitope | pt_BR |
DeCS | Sequência de Aminoácidos | pt_BR |
DeCS | Sequência de Bases | pt_BR |
DeCS | Desenho de Drogas | pt_BR |
DeCS | Mapeamento de Epitopos | pt_BR |
DeCS | Produtos do Gene env | pt_BR |
DeCS | Virus Linfotrópico de Células T Humanas Tipo 1 | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Proteínas Oncogênicas de Retroviridae | pt_BR |
DeCS | Vacinas Virais | pt_BR |