Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/2794
Title: Leishmania amazonensis infection impairs differentiation and function of human dendritic cells
Authors: Favali, Cecilia Beatriz Fiuza
Tavares, Natalia Machado
Clarêncio, Jorge
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. PPGIm. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. PPGIm. Salvador, BA, Brasil
Abstract: Dendritic cells (DCs) are of utmost importance in initiating an immune response and may also function as targets for pathogens. The presence of pathogens inside DCs is likely to impair their functions and thus, influence immune responses. In the present report, we evaluated the impact of the presence of Leishmania amazonensis during differentiation and maturation of human monocyte-derived DCs. The presence of live L. amazonensis parasites during DC differentiation led to a significant decrease in CD80 (92%) and CD1a (56%) expression and an increase in CD86 (56%) cell surface expression. Phenotypic changes were accompanied by a lower secretion of IL-6, observed after 6 days of DC differentiation in the presence of L. amazonensis. DCs differentiated in the presence of L. amazonensis were used as APC in an autologous coculture, and lower amounts of IFN- were obtained compared with control DCs differentiated in the absence of parasites. The effect of heat-killed parasites, but not of Leishmania antigen, during DC differentiation and maturation was similar to that observed with viable parasites. During maturation, the presence of live L. amazonensis parasites, but not of soluble Leishmania antigen, led to a decrease in IL-6 and IL-10 production. In this way, we observed that the parasite is able to abrogate full DC differentiation, causing a delay in the immune response and likely, favoring its establishment in human hosts.
Keywords: Leishmaniasis
Antigen-presenting cells
Cytokines
Costimulatory molecules
T cells
keywords: Leishmaniose
Células que apresentam antígenos
Citocinas
Moléculas co-estimulatórias
Células T
DeCS: Animais
Antígenos de Protozoários
Adesão Celular
Diferenciação Celular
Sobrevivência Celular
Técnicas de Cocultura
Citocinas
Células Dendríticas
Humanos
Interferon gama
Interleucina-6
Leishmania mexicana
Monócitos
Parasitos
Infecções por Protozoários
Solubilidade
Issue Date: 2007
Publisher: Society for Leukocyte Biology
Citation: FAVALI, C. et al. Leishmania amazonensis infection impairs differentiation and function of human dendritic cells. Journal of Leukocyte Biology, v.82, p.1401-1406, dez. 2007.
Description: Agradecimentos a C. Indiani de Oliveira pelos comentários e críticas.
ISSN: 0741-5400
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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