Author | Souza, Jaqueline Gontijo de | |
Author | Fonseca, Flávio Guimarães da | |
Author | Martins Filho, Olindo Assis | |
Author | Carvalho, AndreaTeixeira | |
Author | Martins, Camila Pacheco Silveira | |
Author | Carvalho, Luciana Debortoli | |
Author | Reis, Jordana Grazziela Alves Coelho dos | |
Author | GIPH | |
Author | Stancioli, Edel Figueiredo Barbosa | |
Access date | 2018-07-30T19:19:31Z | |
Available date | 2018-07-30T19:19:31Z | |
Document date | 2010 | |
Citation | SOUZA, Jaqueline Gontijode et al. Diagnostic tool based on an HTLV-1-Tax expression system in eukaryotic cells using a poxvirus vector. Journal of Virological Methods, v. 166, n. 1–2, p. 65-71, 2010. | pt_BR |
ISSN | 0166-0934 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27728 | |
Language | eng | pt_BR |
Publisher | Elsevier Ltd. | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | HTLV-1 | pt_BR |
Subject in Portuguese | HAM/TSP | pt_BR |
Subject in Portuguese | Vacina | pt_BR |
Title | Diagnostic tool based on an HTLV-1-Tax expression system in eukaryotic cells using a poxvirus vector | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jviromet.2010.02.021 | |
Abstract | Human T-lymphotropic virus 1 (HTLV-1) induces an immune-mediated inflammatory disease affecting the nervous system that eventually is accompanied by ocular, rheumatic and dermatologic manifestations (HTLV-1 associated myelopathy/tropical spastic paraparesis, or HAM/TSP). Proviral load and HTLV-1 protein expression, mainly of Tax, is correlated with disease progression and induction of host–virus equilibrium breakdown that, reportedly, involves the presence of Tax-specific cytotoxic T lymphocytes (CTL), T regulatory cells and anti-Tax antibodies. Based on knowledge of anti-Tax antibodies as markers of disease progression, the objectives of this study were both to design an infection/transfection system using the Vaccinia virus and a tax-encoding plasmid for the expression of Tax protein as well as to use this cell support to evaluate anti-Tax IgG by flow cytometry. The flow cytometry assay was standardized using pooled sera from each test group (negative, asymptomatic and HAM/TSP patients). The HAM/TSP group presented higher IgG anti-Tax reactivity (above 70%) than the asymptomatic group (nearly 40% reactivity). The data indicate that the infection/transfection system is useful for assessing Tax expression. This is a promising assay for use as a diagnostic tool to detect IgG anti-Tax and monitor HTLV-1 infected individuals. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil /Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | Tax | pt_BR |
Subject | Vaccinia virus | pt_BR |
Subject | Infection | pt_BR |
Subject | Transfection system | pt_BR |
Embargo date | 2025-01-01 | |