Author | Kohlhoff, Markus | |
Author | Niehues, Anna | |
Author | Wattjes, Jasper | |
Author | Bénéteau, Julie | |
Author | Cord-Landwehr, Stefan | |
Author | El Gueddari, Nour Eddine | |
Author | Bernard, Frank | |
Author | Rivera-Rodriguez, Gustavo R. | |
Author | Moerschbacher, Bruno M. | |
Access date | 2018-07-20T13:14:56Z | |
Available date | 2018-07-20T13:14:56Z | |
Document date | 2017 | |
Citation | KOHLHOFF, Markus et al. Chitinosanase: A fungal chitosan hydrolyzing enzyme with a new and unusually specific cleavage pattern. Carbohydrate Polymers, v. 174, p. 1121-1128, 2017 | pt_BR |
ISSN | 0144-8617 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27609 | |
Language | eng | pt_BR |
Publisher | Elsevier Applied Science Publishers | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Quitosana | pt_BR |
Subject in Portuguese | Hidrolase de quitosana | pt_BR |
Subject in Portuguese | Especificidade do Substrato | pt_BR |
Subject in Portuguese | Impressão digital enzimática | pt_BR |
Subject in Portuguese | Espectrometria de massa | pt_BR |
Title | Chitinosanase: A fungal chitosan hydrolyzing enzyme with a new and unusually specific cleavage pattern. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.carbpol.2017.07.001 | |
Abstract | The biological activities of partially acetylated chitosan oligosaccharides (paCOS) depend on their degree of polymerization (DP), fraction of acetylation (FA), and potentially their pattern of acetylation (PA). Therefore, analyzing structure-function relationships require fully defined paCOS, but these are currently unavailable. A promising approach for obtaining at least partially defined paCOS is using chitosanolytic enzymes. Here we purified and characterized a novel chitosan-hydrolyzing enzyme from the fungus Alternaria alternata possessing an absolute cleavage specificity, yielding fully defined paCOS. It cleaves specifically after GlcN-GlcNAc pairs and is most active towards moderately acetylated chitosans, but shows no activity against fully acetylated or fully deacetylated substrates. These unique properties match neither those of chitinases nor chitosanases. Therefore, the enzyme represents the first member of a new class of chitosanolytic enzymes that will allow for the production of fully defined paCOS. Additionally, it represents a highly valuable tool for fingerprinting analyses of chitosan polymers. | pt_BR |
Affilliation | Fundacao Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Affilliation | Institute for Biology and Biotechnology of Plants. University of Muenster. Schlossplatz 8, 48143 Münster, Germany | pt_BR |
Subject | Chitosan | pt_BR |
Subject | Chitosan hydrolase | pt_BR |
Subject | Substrate specificity | pt_BR |
Subject | Enzymatic fingerprinting | pt_BR |
Subject | Mass spectrometry | pt_BR |
Embargo date | 2025-01-01 | |