Author | Ribeiro, R. L. | |
Author | Machry, L. | |
Author | Brazil, J. M. V. | |
Author | Ramos, T. M. V. | |
Author | Avelar, K. E. S. | |
Author | Pereira, M. M. | |
Access date | 2018-07-17T15:36:06Z | |
Available date | 2018-07-17T15:36:06Z | |
Document date | 2009 | |
Citation | RIBEIRO, R. L. et al. Technical improvement to prevent DNA degradation of Leptospira spp. in pulsed field gel electrophoresis. Letters in Applied Microbiology, v.49, ´.289-291, 2009. | pt_BR |
ISSN | 0266-8254 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27569 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Leptospirose | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | tiourea | pt_BR |
Subject in Portuguese | Degradação do DNA | pt_BR |
Subject in Portuguese | Leptospira | pt_BR |
Title | Technical improvement to prevent DNA degradation of Leptospira spp. in pulsed field gel electrophoresis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/j.1472-765X.2009.02641.x | |
Abstract | Leptospirosis is a public health problem. Infection with pathogenic Leptospira occurs by exposure to many environments and is traditionally associated with occupational risk activities. Pulsed-field gel electrophoresis was used to investigate the epidemiological relatedness among Leptospira isolates. However, analysis by PFGE yielded inconclusive data as a result of extensive DNA degradation. This degradation can be significantly reduced by the inclusion of thiourea in the electrophoresis buffer, improving the analysis of DNA banding patterns. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia.. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia.. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bacteriologia.. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia.. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia.. Centro Colaborador de WHO/PAHO. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | DNA degradation | pt_BR |
Subject | infection | pt_BR |
Subject | Leptospira | pt_BR |
Subject | PFGE | pt_BR |
Subject | thiourea | pt_BR |
e-ISSN | 1472-765X | |
Embargo date | 2030-01-01 | |