Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/2755
Title: Characterization of the vascular changes in schistosomal portal (pipestem) fibrosis of mice
Authors: Silva, Luciana Menezes da
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Andrade, Zilton de Araújo
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquis Gonçalo Moniz. Laboratórios de Patologia Experimental. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratórios de Patologia Experimental. Salvador, BA, Brasil
Abstract: Mice chronically infected with Schistosoma mansoni develop one of two anatomical forms of hepatic lesions: severe, periportal (pipestem) fibrosis or milder, isolated granulomas. The pathogenesis of periportal fibrosis is poorly understood. In this work we compared mice with either periportal fibrosis or isolated granulomas to identify specific markers of severe pathology. BALB/c or Swiss Webster mice were infected with 30 cercarie, once or six times, and liver biopsies were performed to classify the animals into two pathological groups 16 weeks later. Sixty percent of the animals sacrificed at 20 or 24 weeks had periportal fibrosis, 15–20% had isolated granulomas and the remainder had indeterminate pathology. There was no correlation between frequency of infections or egg burden (eggs/g liver) at 20 and 24 weeks post-infection and the development of periportal fibrosis. Livers with periportal fibrosis at 20 or 24 weeks of infection were characterized by larger areas of fibrotic tissue and greater vascularization compared to livers of mice with isolated granulomas. Plastic casts of the portal vein system showed marked changes in vascular structure in mice with periportal fibrosis, including collateral vessels sprouting from the main portal branches, amputation of the more delicate peripheral ramifications, and distortions of the medium and small branches. Vascular changes were not observed in mice with isolated granulomas. These results suggest that the interaction between schistosome eggs and portal vascular changes is of paramount importance in the development of pipestem fibrosis.
Keywords: Schistosoma mansoni
Periportal (pipestem) Fibrosis
Periovular Granulomas
Hepatic Fibrosis
keywords: Schistosoma mansoni
Fibrose Periportal
Granuloma Periovular
Fibrose Hepática
DeCS: Schistosoma mansoni
Fibrose
Granuloma
Cirrose Hepática
Issue Date: 2006
Citation: SILVA, L. M. et al. Characterization of the vascular changes in schistosomal portal (pipestem) fibrosis of mice. Acta Tropica, v. 98, n. 1, p.34-42, 2006.
Description: Agradecimentos a Sheilla Andrade de Oliveira, Rejane M.C. Menezes, Antonio Carlos S. Santos e Ana Cristina Gonzalez pelo apoio técnico e Brendan Flannery pela revisão do manuscrito.
DOI: 10.1016/j.actatropica.2006.01.011
ISSN: 0001-706X
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

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