Author | Cordeiro, Marli Tenório | |
Author | Braga-Neto, Ulisses | |
Author | Nogueira, Rita Maria Ribeiro | |
Author | Marques, Ernesto T. A. | |
Access date | 2018-07-11T13:12:25Z | |
Available date | 2018-07-11T13:12:25Z | |
Document date | 2009 | |
Citation | CORDEIRO, M. T. et al. Reliable classifier to differentiate primary and secondary acute dengue infection based on IgG ELISA. PloS One, v. 4, n. 4, p. e4945, 2009. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27466 | |
Sponsorship | Os autores gostariam de agradecer o apoio do Instituto Nacional de Alergia e Doenças Infecciosas (NIAID / NIH), sob as concessões U19 AI56541 e N01 AI 40085. O Dr. Braga-Neto gostaria de agradecer o apoio da Pesquisa Nacional Brasileira. Conselho Nacional (CNPq), no âmbito do Subsídio DCR 35.0382 / 2004.2. Os financiadores não tiveram nenhum papel no desenho do estudo, coleta e análise de dados, decisão de publicar ou preparação do manuscrito. | pt_BR |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Dengue | pt_BR |
Subject in Portuguese | PCR | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Title | Reliable classifier to differentiate primary and secondary acute dengue infection based on IgG ELISA | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0004945 | |
Abstract | Dengue virus infection causes a wide spectrum of illness, ranging from sub-clinical to severe disease. Severe dengue is associated with sequential viral infections. A strict definition of primary versus secondary dengue infections requires a combination of several tests performed at different stages of the disease, which is not practical.
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Methods and Findings
We developed a simple method to classify dengue infections as primary or secondary based on the levels of dengue-specific IgG. A group of 109 dengue infection patients were classified as having primary or secondary dengue infection on the basis of a strict combination of results from assays of antigen-specific IgM and IgG, isolation of virus and detection of the viral genome by PCR tests performed on multiple samples, collected from each patient over a period of 30 days. The dengue-specific IgG levels of all samples from 59 of the patients were analyzed by linear discriminant analysis (LDA), and one- and two-dimensional classifiers were designed. The one-dimensional classifier was estimated by bolstered resubstitution error estimation to have 75.1% sensitivity and 92.5% specificity. The two-dimensional classifier was designed by taking also into consideration the number of days after the onset of symptoms, with an estimated sensitivity and specificity of 91.64% and 92.46%. The performance of the two-dimensional classifier was validated using an independent test set of standard samples from the remaining 50 patients. The classifications of the independent set of samples determined by the two-dimensional classifiers were further validated by comparing with two other dengue classification methods: hemagglutination inhibition (HI) assay and an in-house anti-dengue IgG-capture ELISA method. The decisions made with the two-dimensional classifier were in 100% accordance with the HI assay and 96% with the in-house ELISA.
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Conclusions
Once acute dengue infection has been determined, a 2-D classifier based on common dengue virus IgG kits can reliably distinguish primary and secondary dengue infections. Software for calculation and validation of the 2-D classifier is made available for download. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / Secretaria de Saúde do Estado de Pernambuco. Laboratório Central de Saúde Pública. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / Texas A&M University. College Station. Department of Electrical and Computer Engineering. Texas, United States of America. | pt_BR |
Affilliation | Fiocruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / The Johns Hopkins School of Medicine. Division of Infectious Diseases. Department of Medicine. Baltimore, Maryland, United States of America / The Johns Hopkins School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
Subject | Dengue | pt_BR |
Subject | PCR | pt_BR |
Subject | Infection | pt_BR |
DeCS | Doença Aguda | pt_BR |
DeCS | Dengue / classificação | pt_BR |
DeCS | Dengue / imunologia | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Fêmea | pt_BR |
DeCS | Testes de hemaglutinação | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Imunoglobulina G / imunologia | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Padrões de referência | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |