Author | Saraiva, Karina Lidianne Alcântara | |
Author | Soares e Silva, Amanda Karolina | |
Author | Wanderley, Maria Inês | |
Author | Araújo, Araken Almeida de | |
Author | Souza, José Roberto Botelho de | |
Author | Peixoto, Christina Alves | |
Access date | 2018-07-11T11:14:18Z | |
Available date | 2018-07-11T11:14:18Z | |
Document date | 2009 | |
Citation | SARAIVA, K. L. A. et al. Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion. International Journal of Experimental Pathology, v. 90, n. 4, p. 454-462, Aug. 2009. | |
ISSN | 1365-2613 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27464 | |
Sponsorship | Este trabalho foi apoiado pela Fundação Oswaldo Cruz (FIOCRUZ) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células de Leydig | |
Subject in Portuguese | P450scc | |
Subject in Portuguese | Sildenafil | |
Subject in Portuguese | StAR | |
Subject in Portuguese | Esteroidogênese | |
Subject in Portuguese | Testosterona | |
Title | Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion | pt_BR |
Type | Article | en |
DOI | 10.1111/j.1365-2613.2009.00660.x | |
Abstract | The phosphodiesterase type 5 (PDE5) inhibitor, Sildenafil, is a novel, oral treatment approach for pulmonary hypertension. As Leydig cells present PDE5, this study was conducted to investigate the effects of the chronic treatment with Sildenafil (25 mg/kg) on male Swiss Webster mice steroidogenesis. After a 4-week long experimental design, Leydig cells were analysed by morphological and immunocytochemical procedures. Serum testosterone was assayed by radioimmunoassay. Leydig cells presented noteworthy ultrastructural alterations, such as a vesicular smooth endoplasmic reticulum, large vacuoles scattered through the cytoplasm, enlarged mitochondria with discontinue cristaes and whorle membranes with vesicles at the periphery, which are typical characteristics of an activated steroid-secreting cell. Important immunocytochemical labelling for steroidogenic acute regulatory protein, cytochrome P450 side-chain cleavage enzyme and testosterone were detected in isolated Leydig cells. In addition, Sildenafil-treated mice showed significant increased levels of total testosterone. The results obtained in the present study are consistent with the hypothesis that the accumulation of cyclic guanosine monophosphate by PDE5 inhibition could be involved in the androgen biosynthesis stimulation. Important clinical implications of hormonal disorders should be taken into account for patients with pulmonary hypertension. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil / Centro de Tecnologias Estratégicas do Nordeste. Recife, PE, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil. | |
Affilliation | Universidade Federal de Pernambuco. Departamento de Fisiologia e Farmacologia. Recife, PE, Brasil. | |
Affilliation | Universidade de Pernambuco. Faculdade de Ciências Médicas. Recife, PE, Brasil. | |
Affilliation | Universidade Federal de Pernambuco. Departamento de Zoologia. Recife, PE, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil / Centro de Tecnologias Estratégicas do Nordeste. Recife, PE, Brasil. | |
Subject | Leydig cells | |
Subject | P450scc | |
Subject | Sildenafil | |
Subject | StAR | |
Subject | Steroidogenesis | |
Subject | Testosterone | |
DeCS | Células de Leydig / metabolismo | |
DeCS | Inibidores da Fosfodiesterase 5 | |
DeCS | Piperazinas / farmacologia | |
DeCS | Sulfonas / farmacologia | |
DeCS | Testosterona / biossíntese | |
DeCS | Agentes vasodilatadores / farmacologia | |
Embargo date | 2020-01-01 | |