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https://www.arca.fiocruz.br/handle/icict/27091
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ArticleCopyright
Open access
Embargo date
2019-02-28
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- INI - Artigos de Periódicos [3397]
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A RAPID AND SIMPLE HPLC METHOD FOR THERAPEUTIC MONITORING OF VANCOMYCIN
Author
Affilliation
Faculty of Pharmaceutical Sciences, University of São Paulo. São Paulo - SP
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.
Faculty of Pharmacy, Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Hospital Infection Control Team, University Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz.Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.
Faculty of Pharmacy, Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Hospital Infection Control Team, University Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz.Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Abstract
Therapeutic monitoring of the antibiotic vancomycin is important to achieve specific plasma concentration and prevent toxic effects. Several assays have been described for vancomycin determination in clinical practice, but high-performance liquid chromatography is still considered the gold standard for the quantification of vancomycin. In this study, we developed a new and rapid high-performance liquid chromatography method requiring 50 μL of plasma for the quantification of vancomycin. Acetonitrile was used for processing plasma by protein precipitation (1:2.5). Isocratic chromatographic analysis was carried out on a C18 silica-based (2.7 μm) column with the mobile phase containing 20 mM ammonium acetate/formic acid buffer (pH 4.0):methanol 88:12 (v/v). A diode array detector was used for UV detection at 240 nm. This method was validated according to the Brazilian Health Surveillance Agency legislation and International Conference on Harmonization guidelines. The measurement range was 1-100 μg/mL, analysis time was 8 min, and intermediate precision was <12%, supporting the present method as a fast, simple, and effective alternative for therapeutic monitoring of vancomycin.
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