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2023-01-01
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ETIOLOGICAL AGENTS OF VIRAL MENINGITIS IN CHILDREN FROM A DENGUE-ENDEMIC AREA, SOUTHEAST REGION OF BRAZIL
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Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil /Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Medicina de Diamantina. Diamantina, MG, Brazil
Fundação Hospitalar do Estado de Minas Gerais. Hospital Infantil João Paulo II. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Center for Biologics Research and Review. Food and Drug Administration. Silver Spring, MD, USA
Fundação Oswaldo Cruz. Instituto René Rachou. Neurogenômica. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Fundação Hospitalar do Estado de Minas Gerais. Hospital Infantil João Paulo II. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Center for Biologics Research and Review. Food and Drug Administration. Silver Spring, MD, USA
Fundação Oswaldo Cruz. Instituto René Rachou. Neurogenômica. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Abstract
Meningitis is a disease with a global distribution that constitutes aworldwide burden,with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aimof this workwas to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. CSF of 70 children with the presumptive diagnosis of viral meningitis was analyzed by real time PCR (qPCR). Viruses were identified by qPCR in 44 CSF samples (62.9%).
Among them, 31 were identified as Enterovirus (ENTV) (70.4%), six as Human herpes virus 3 (HHV-3) (13.6%), five as Dengue virus (DENV) (11.7%), one as Human herpes virus 1–2 (2.3%) and one as Human herpes virus 5 (2.3%). Patients in the HHV-positive groups had increased percentage of polymorphonuclear neutrophils (PMN) (mean of 81%) while the groups of patients with DENV and ENTV had a mean of 30.9%.
This study contributes to the knowledge of the epidemiological distribution of viral agents in CNS infections in children. In addition, it raises the relevance of DENV as an agent of CNS infection, and reinforces the importance for molecular in the cases of CNV infection.
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