Author | Matos, Natália Alves de | |
Author | Silva, Josiane Fernandes da | |
Author | Matsui, Tamires Cardoso | |
Author | Damasceno, Karine Araujo | |
Author | Duarte, Igor Dimitri Gama | |
Author | Lemos, Virgínia Soares | |
Author | Cassali, Geovanni D | |
Author | Klein, André | |
Access date | 2018-06-13T17:32:32Z | |
Available date | 2018-06-13T17:32:32Z | |
Document date | 2013 | |
Citation | Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2 | pt_BR |
ISSN | 0360-3997 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/26877 | |
Description | Damasceno, Karine Araújo. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | Conselho Nacional de Pesquisa e Desenvolvimento (CNPq/Brazil), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG/Brazil) and CAPES/Brazil. | pt_BR |
Language | eng | pt_BR |
Publisher | Springer Verlag | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Eosinófilos | pt_BR |
Subject in Portuguese | Migração de eosinófilos | pt_BR |
Subject in Portuguese | Triptase de mastócitos | pt_BR |
Subject in Portuguese | PAR-2 | pt_BR |
Subject in Portuguese | Pleurisia | pt_BR |
Title | Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s10753-013-9664-5 | |
Abstract | Proteinase-activated receptor (PAR) 2 has been implicated in eosinophil migration. Mast cell (MC) tryptase has been similarly implicated in allergic diseases through the activation of PAR-2, but the role of this receptor in MC tryptase-induced inflammation is not well elucidated. This study aims to investigate the ability of MC tryptase or PAR-2 activating peptide (SLIGRL-NH2) to induce eosinophil recruitment to the pleural cavity of mice. Mast cell tryptase-injected mice were pretreated with PAR-2 antagonist ENMD-1068. Mice injected with SLIGRL-NH2 were pretreated with mast cell tryptase inhibitor APC 366, and eosinophil migration into the pleural cavity and PAR-2 expression was analyzed after 24 or 48 h. SLIGRL-NH2-induced eosinophil recruitment was inhibited by APC 366, and MC tryptase-induced eosinophil recruitment was abolished by ENMD-1068. MC tryptase induced PAR-2 expression on pleural eosinophils. Our results demonstrate a key role for PAR-2 in mediating eosinophil recruitment in MC tryptase-induced pleurisy in mice. The ability of MC tryptase to inducing PAR-2 expression on eosinophils corroborates the relevance of MC tryptase and PAR-2 on modulating eosinophil migration. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Farmacologia. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Eosinophils | pt_BR |
Subject | Eosinophil migration | pt_BR |
Subject | Mast cell tryptase | pt_BR |
Subject | PAR-2 | pt_BR |
Subject | Pleurisy | pt_BR |