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https://www.arca.fiocruz.br/handle/icict/26874
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2022-01-01
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DIFFERENCES IN BACTERIAL COMPOSITION BETWEEN MEN'S AND WOMEN'S RESTROOMS AND OTHER COMMON AREAS WITHIN A PUBLIC BUILDING
Author
Dobbler, Priscila Caroline Thiago
Laureano, Álvaro Macedo
Sarzi, Deise Schroder
Cañón, Ehidy Rocio Peña
Metz, Geferson Fernando
Freitas, Anderson Santos de
Takagaki, Beatriz Midori
D´Oliveira, Cristiane Barbosa
Pylro, Victor Satler
Copetti, André Carlos
Victoria, Filipe
Redmile-Gordon, Marc
Morais, Daniel Kumazawa
Roesch, Luiz Fernando Wurdig
Laureano, Álvaro Macedo
Sarzi, Deise Schroder
Cañón, Ehidy Rocio Peña
Metz, Geferson Fernando
Freitas, Anderson Santos de
Takagaki, Beatriz Midori
D´Oliveira, Cristiane Barbosa
Pylro, Victor Satler
Copetti, André Carlos
Victoria, Filipe
Redmile-Gordon, Marc
Morais, Daniel Kumazawa
Roesch, Luiz Fernando Wurdig
Affilliation
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade de São Paulo. Escola de Agricultura Luiz de Queiroz. Departamento de Ciências do Solo. Laboratório de Microbiologia do Solo. Piracicaba, SP, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Department of Sustainable Soils and Grassland Systems. Rothamsted Research. Harpenden, Herts AL5 2JQ, UK
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica e Informatica de Biosistema. Belo Horizonte, MG, Brasil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade de São Paulo. Escola de Agricultura Luiz de Queiroz. Departamento de Ciências do Solo. Laboratório de Microbiologia do Solo. Piracicaba, SP, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Department of Sustainable Soils and Grassland Systems. Rothamsted Research. Harpenden, Herts AL5 2JQ, UK
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica e Informatica de Biosistema. Belo Horizonte, MG, Brasil
Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia. Sao Gabriel, RS, Brazil
Abstract
Humans distribute a wide range of microorganisms around building interiors, and some of these are potentially pathogenic. Recent research established that humans are the main drivers of the indoor microbiome and up to now significant literature has been produced about this topic. Here we analyzed differences in bacterial composition between men’s and women’s restrooms and other common areas within the same public building. Bacterial DNA samples were collected from restrooms and halls of a three-floor building from the Federal University of Pampa, RS, Brazil. The bacterial community was characterized by amplification of the V4 region of the 16S rRNA gene and sequencing. Throughout all samples, the most abundant phylum was Proteobacteria, followed by Actinobacteria, Bacteroidetes and Firmicutes. Beta diversity metrics showed that the structure of the bacterial communities were different among the areas and floors tested, however, only 6–9% of the variation in bacterial communities was explained by the area and floors sampled. A few microorganisms showed significantly differential abundance between men’s and women’s restrooms, but in general, the bacterial communities from both places were very similar. Finally, significant differences among the microbial community profile from different floors were reported, suggesting that the type of use and occupant demographic within the building may directly influence bacterial dispersion and establishment.
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