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https://www.arca.fiocruz.br/handle/icict/26816
ROLE OF FAT-STORING CELLS IN SCHISTOSOMAL HEPATIC FIBROSIS OF MICE
Schistosoma mansoni
Fibrose hepática
Hipervitaminose A
Granulomas esquistossomóticos
Schistosoma mansoni-
Liver fibrosis
Hypervitaminosis A
Schistosomal granulomas
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
University of Bonn. Department of Pathology. Germany
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
University of Bonn. Department of Pathology. Germany
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract
The involvement of fat-storing cells (FSC)
in hepatic schistosomal granuloma was investigated in
mice infected with Schistosoma mansoni cercariae. After
infection, 24 animals were treated with s.c. injections of
vitamin A in a total dose of 210,000 IU given twice a
week for 3 weeks. Two other groups of 24 animals each
were: a) non-infected vitamin A-treated animals and (b)
untreated infected controls. Animals from all groups
were killed at weekly intervals from the 5th through
the 10th week following infection. Pieces of liver were
examined by light microscopy and transmission electron
microscopy. In all vitamin A-treated animals, FSC disclosed
prominent cytoplasmic fat droplets, which permitted
their prompt identification in the light and in
the electron microscope. They were found in large
numbers as a constituent of periovular granulomas. In
infected controls, FSC were not identified in granulomas,
possibly because lipid droplets disappeared during
differentiation to the fibroblastic phenotype. FSC also
appeared within areas of septal fibrosis. These data suggest
that FSC play an important part in focal portohepatic
fibrosis during granuloma formation around
S. mansoni eggs in the liver of mice.
Keywords in Portuguese
Células armazenadoras de gorduraSchistosoma mansoni
Fibrose hepática
Hipervitaminose A
Granulomas esquistossomóticos
Keywords
Fat-storing cellsSchistosoma mansoni-
Liver fibrosis
Hypervitaminosis A
Schistosomal granulomas
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