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2050-01-01
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LOSS OF GENETIC DIVERSITY IN CULEX QUINQUEFASCIATUS TARGETED BY A LYMPHATIC FILARIASIS VECTOR CONTROL PROGRAM IN RECIFE, BRAZIL
Bacillus sphaericus
Filariose linfática
Microssatélites
Desvio genético
Gene receptor cqm1
Bacillus sphaericus
Lymphatic filariasis
Microsatellite
Genetic drift
Receptor gene cqm1
Brasil / epidemiologia
Culex / genética
Filariose Linfática / epidemiologia
Elefantíase, filarial / prevenção & controle
Freqüência Genética
Deriva Genética
Variação genética
Humanos
Repetições de Microssatélites
Controle de Mosquito
Mutação
Densidade populacional
Affilliation
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, UK.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, UK.
Abstract
Recife is one of the largest cities in north-eastern Brazil and is endemic for lymphatic filariasis transmitted by Culex quinquefasciatus. Since 2003 a control program has targeted mosquito larvae by elimination of breeding sites and bimonthly application of Bacillus sphaericus. To assess the impact of this program on the local vector population we monitored the genetic diversity and differentiation of Cx. quinquefasciatus using microsatellites and a B. sphaericus-resistance associated mutation (cqm1(REC)) over a 3-year period. We detected a significant but gradual decline in allelic diversity, which, coupled with subtle temporal genetic structure, suggests a major impact of the control program on the vector population. Selection on cqm1(REC) does not appear to be involved with loss of neutral diversity from the population, with no temporal trend in resistant allele frequency and no correlation with microsatellite differentiation. The evidence for short-term genetic drift we detected suggests a low ratio of effective population size: census population size for Cx. quinquefasciatus, perhaps coupled with strong geographically-restricted population structure. Spatial definition of populations will be an important step for success of an expanded vector control program.
Keywords in Portuguese
Culex quinquefasciatusBacillus sphaericus
Filariose linfática
Microssatélites
Desvio genético
Gene receptor cqm1
Keywords
Culex quinquefasciatusBacillus sphaericus
Lymphatic filariasis
Microsatellite
Genetic drift
Receptor gene cqm1
DeCS
AnimaisBrasil / epidemiologia
Culex / genética
Filariose Linfática / epidemiologia
Elefantíase, filarial / prevenção & controle
Freqüência Genética
Deriva Genética
Variação genética
Humanos
Repetições de Microssatélites
Controle de Mosquito
Mutação
Densidade populacional
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