Author | Santos, Maria Isabel Magalhães Andrade dos | |
Author | Pacheco, Sidelcina Rugieri | |
Author | Stocker, Andreas | |
Author | Schinoni, Maria Isabel | |
Author | Paraná, Raymundo | |
Author | Reis, Mitermayer Galvão dos | |
Author | Silva, Luciano Kalabric | |
Access date | 2018-04-03T18:24:48Z | |
Available date | 2018-04-03T18:24:48Z | |
Document date | 2017 | |
Citation | SANTOS, M. I. M. A. et al. Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection. Journal of Medical Virology, v. 89, p. 1811–1816, 2017. | pt_BR |
ISSN | 0146-6615 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25599 | |
Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico - Universal-MCTI/ CNPq, Grant number: process: 478322/2012-7; Instituto Gonçalo Moniz - FIOCRUZ– BA/BRAZIL, Grant number: 14/2012 | pt_BR |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | HBV | pt_BR |
Subject in Portuguese | Vírus da hepatite B | pt_BR |
Subject in Portuguese | Análogos nucleos (t) ide | pt_BR |
Subject in Portuguese | resistência | pt_BR |
Title | Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1002/jmv.24853 | |
Abstract | The Brazilian public health system (SUS) has provided antiviral drugs for chronic hepatitis B treatment for over 10 years, but a system for monitoring for drug-related resistance mutations is not available. Determine the presence of HBV mutations associated with resistance to nucleos(t)ide analogs among 81 patients with chronic HBV infection in Salvador-BA-Brazil. HBV-DNA was PCR amplified with primers deduced from the rt domain at the HBV P gene, the sequence extended 1032 bp (from amino acid 1 to 344-rt domain). Those sequences were submitted to the HBV drug resistance database to retrieve each mutation according to the genotype. HBV genotype A1 (85.2%) was the most prevalent, followed by genotype A2 (4.9%), F (6.2%), and C1, D2, and D4 (1.2% each). Six patients (7%) exhibited resistance mutations to LAM, ETV, and TDF: two with patterns L180M + M204V and four with other different patterns: L80I + L180M + M204I; L80V + L180M + M204V; M204I; A194T. All of these mutations were present in patients with genotype A (four A1 and two A2). In addition, four mutations in gene S (three cases with the sI195M mutation and one with the W196L mutation), were detected, corresponding to a rate of 6% of vaccine escape mutations. Althougth the small sample size, an association was found between the occurrence of HBV resistance mutations and HBeAg positivity, co-infection with HIV and a history of treatment for HBV and/or HIV. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Laboratório do Serviço de Gastro-Hepatologia de Pesquisa em Infectologia. Salvador, BA,Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Ambulatório Magalhães Neto. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Ambulatório Magalhães Neto. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | HBV | pt_BR |
Subject | Hepatitis B virus | pt_BR |
Subject | Nucleos(t)ides analogs | pt_BR |
Subject | Resistance | pt_BR |