Author | Silva, Cleidson Manoel Gomes da | |
Author | Castro, Simone Vieira | |
Author | Faustino, Luciana Rocha | |
Author | Rodrigues, Giovanna Quintino | |
Author | Brito, Ivina Rocha | |
Author | Rossetto, Rafael | |
Author | Saraiva, Márcia Viviane Alves | |
Author | Campello, Claudio Cabral | |
Author | Lobo, Carlos Henrique | |
Author | Souza, Carlos Eduardo Azevedo | |
Author | Moura, Arlindo de Alencar Araripe | |
Author | Donato, Mariana Aragão Matos | |
Author | Peixoto, Christina Alves | |
Author | Figueiredo, José Ricardo de | |
Access date | 2018-03-28T15:37:17Z | |
Available date | 2018-03-28T15:37:17Z | |
Document date | 2015 | |
Citation | DA SILVA, C. M. G. et al. Activin-A promotes the development of goat isolated secondary follicles in vitro. Zygote (Cambridge, England), v. 23, n. 1, p. 41–52, fev. 2015. | pt_BR |
ISSN | 1469-8730 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25545 | |
Sponsorship | Esta pesquisa contou com o apoio de doações do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasil (RENORBIO: bolsa número 554812 ⁄ 2006–1), Financiadora de Estudos e Projetos (FINEP) e Pronex – Brasil. Cleidson Manoel Gomes da Silva é bolsista da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil. | pt_BR |
Language | eng | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Activina-A | pt_BR |
Subject in Portuguese | Bode | pt_BR |
Subject in Portuguese | Cultura in vitro | pt_BR |
Subject in Portuguese | Aromatase P450 | pt_BR |
Subject in Portuguese | Folículo secundário | pt_BR |
Title | Activin-A promotes the development of goat isolated secondary follicles in vitro | pt_BR |
Type | Article | pt_BR |
DOI | 10.1017/S0967199413000294 | |
Abstract | The role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥ 150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cultures with added activin-A than in the cultured control (P < 0.05). The addition of 50 ng/ml activin-A increased the follicular growth rate in the final third of the culture (days 12-18), resulting in a higher percentage of meiosis resumption (P < 0.05). On day 6, the addition of activin-A (50 ng/ml) increased the levels of ActR-IA mRNA compared with the cultured control (P < 0.05). After 18 days, the addition of 50 ng/ml activin-A significantly increased the levels of its own mRNA compared with the non-cultured control. Moreover, this treatment reduced the mRNA levels of P450 aromatase in comparison with the cultured control (P < 0.05). Higher levels of P450 aromatase mRNA were found for both activin-A treatments compared with the non-cultured control (P < 0.05). No difference in estradiol levels was detected among any of the tested treatments. In conclusion, the addition of activin-A to culture medium stimulated early antrum formation as well as an increase in the daily follicular growth rate and the percentage of meiosis resumption. | pt_BR |
Affilliation | Universidade Estadual do Ceará (UECE). Programa de Pós-Graduação em Ciências Veterinárias (PPGCV). Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA). Fortaleza, CE, Brasil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil. | pt_BR |
Affilliation | State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil. | pt_BR |
Subject | Activin-A | pt_BR |
Subject | Goat | pt_BR |
Subject | In vitro culture | pt_BR |
Subject | P450 aromatase | pt_BR |
Subject | Secondary follicle | pt_BR |
DeCS | Receptores de Activina Tipo I / genética | pt_BR |
DeCS | Receptores de Activina Tipo II / genética | pt_BR |
DeCS | Activins / genética | pt_BR |
DeCS | Activins / farmacologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Aromatase / genética | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Estradiol / análise | pt_BR |
DeCS | Estradiol / secreção | pt_BR |
DeCS | Fêmea | pt_BR |
DeCS | Regulação da expressão gênica / efeitos de drogas | pt_BR |
DeCS | Cabras | pt_BR |
DeCS | Técnicas de maturação de ovócitos in vitro / métodos | pt_BR |
DeCS | Folículo Ovariano / efeitos de drogas | pt_BR |
DeCS | Folículo Ovariano / fisiologia | pt_BR |
DeCS | Folículo Ovariano / ultraestrutura | pt_BR |
DeCS | Receptores FSH / genética | pt_BR |
Embargo date | 2050-01-01 | |