Author | Victoria, Matías | |
Author | Guimarães, Flávia Ramos | |
Author | Fumian, Tulio Machado | |
Author | Ferreira, Fabiana Fioretti Martins | |
Author | Vieira, Carmen Baur | |
Author | Shubo, Tatsuo | |
Author | Leite, José Paulo Gagliardi | |
Author | Miagostovih, Marise Pereira | |
Access date | 2018-03-20T13:15:04Z | |
Available date | 2018-03-20T13:15:04Z | |
Document date | 2010 | |
Citation | VICTORIA, Matías; et al. One year monitoring of norovirus in a sewage treatment plant in Rio de Janeiro, Brazil. Journal of Water and Health, v.08.1, p.158-165, 2010. | pt_BR |
ISSN | 1477-8920 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25415 | |
Language | eng | pt_BR |
Publisher | IWA Publishing | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Norovirus | pt_BR |
Subject in Portuguese | Eficiência de tratamento | pt_BR |
Subject in Portuguese | esgoto | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Subject in Portuguese | gastroenterite aguda | pt_BR |
Title | One year monitoring of norovirus in a sewage treatment plant in Rio de Janeiro, Brazil | pt_BR |
Type | Article | pt_BR |
Abstract | Norovirus (NoV) is one of the most important aetiological agents of acute gastroenteritis both in
developed and developing countries. NoV is shed in high concentrations by infected persons and
contaminates recreational and drinking water through sewage discharge into the environment.
The aim of this study was to determine the prevalence, genotypes and removal ratio of NoV by
PCR, seminested-PCR and quantitative PCR (qPCR) assays in a sewage treatment plant in Rio de
Janeiro city, Brazil, during one year of surveillance. NoV was detected in 7 (15%), 14 (29%) and 28
(58%) samples using PCR, seminested-PCR and qPCR, respectively. The mean removal ratio for
the activated sludge process was 0.6 log10 for NoV genogroup I (GI) and 0.32 log10 for NoV
genogroup II (GII). The peak NoV concentration was detected in the coldest months, with 53,300
genomic copies/litre. Nucleotide sequencing and phylogenetic analysis revealed that five strains
clustered with GI strains and six with GII strains. This study demonstrates that NoV spreads into
the environment despite the sewage treatment process and remains a source of waterborne
outbreaks of acute gastroenteritis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Conselho Consultivo Técnico de Infra-estrutura e Meio Ambiente. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Norovirus | pt_BR |
Subject | quantitative PCR | pt_BR |
Subject | sewage | pt_BR |
Subject | treatment efficiency | pt_BR |
Subject | acute gastroenteritis | pt_BR |
Embargo date | 2030-01-01 | |