Author | Pires, Ana Clara Araújo Machado | |
Author | Villegas, Luís Eduardo Martinez | |
Author | Campolina, Thais Bonifácio | |
Author | Orfanó, Alessandra Silva | |
Author | Pimenta, Paulo Filemon Paolucci | |
Author | Secundino, Nágila Francinete Costa | |
Access date | 2018-03-05T15:48:16Z | |
Available date | 2018-03-05T15:48:16Z | |
Document date | 2017 | |
Citation | PIRES, Ana Clara Araújo Machado et al. Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis. Parasit Vectors, v. 10, n. 1, p. 1-9, 2017. | pt_BR |
ISSN | 1756-3305 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25058 | |
Language | eng | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Lutzomyia longipalpis | pt_BR |
Title | Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13071-017-2593-7 | |
Abstract | Background: The leishmaniases are a group of diseases caused by protozoans of the genus Leishmania, which are transmitted by the bite of phlebotomine sand flies. In the New World, Lutzomyia longipalpis is the most important vector of visceral leishmaniasis and is a proven vector for Leishmania infantum chagasi in Brazil. During development within the vector, Leishmania can interact with a variety of microorganisms such as fungi and bacteria. The presence of bacteria in the midgut of sand flies can influence the development and survival of the parasite.
Results: The bacteria-targeted metagenomic analysis revealed different community compositions between the distinct physiological stages of those tested. The amplicon-oriented metagenomic profiling revealed 64 bacterial genera and 46 families. By crossing the taxa indices from each experimental condition a core composed of 6 genera was identified (Enterobacter, Serratia, Stenotrophomonas, Enhydrobacter, Pseudomonas and Chryseobacterium).
Conclusions: The observed dynamic nature of the bacterial community expands the knowledge pertaining to the tripartite host-microbiota-pathogen interactions. Further studies addressing how laboratory and field collected communities differ are critical to successfully develop control strategies based on bacterial symbionts and paratransgenesis, as already tested in other arthropod vectors. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | Wild-caught | pt_BR |
Subject | Lutzomyia longipalpis | pt_BR |
Subject | Metagenomic analysis | pt_BR |
Subject | Microbiota | pt_BR |
Subject | Leishmania | pt_BR |