Author | Campos, Taís Menezes Cerqueira | |
Author | Passos, Sara Timoteo | |
Author | Novais, Fernanda Oliveira | |
Author | Beiting, Daniel P | |
Author | Costa, Rúbia Suely Santana | |
Author | Queiroz, Adriano | |
Author | Mosser, David | |
Author | Scott, Phillip | |
Author | Carvalho, Edgar Marcelino de | |
Author | Carvalho, Lucas Pedreira de | |
Access date | 2018-02-21T17:09:59Z | |
Available date | 2018-02-21T17:09:59Z | |
Document date | 2014 | |
Citation | CAMPOS, T. M. C. et al. Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 8, n. 11, e3282, 2014. | pt_BR |
ISSN | 1935-2727 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/24899 | |
Description | CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | National Institutes of Health [grant AI088650] and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e
Tecnologia – Doenças Tropicais [grant 573839/2008-5]. | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose Cutânea | pt_BR |
Subject in Portuguese | Monócitos | pt_BR |
Subject in Portuguese | Fator de necrose tumoral alfa | pt_BR |
Subject in Portuguese | Leucócitos mononucleares | pt_BR |
Subject in Portuguese | Matrix Metalloproteinase 9 | pt_BR |
Subject in Portuguese | Humanos | pt_BR |
Title | Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0003282 | |
Abstract | Cutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous population subdivided into classical, intermediate and non-classical, and are known to migrate to inflammatory sites and secrete inflammatory mediators. TNF participates in the induction of matrix metalloproteinases (MMPs). MMP-9 is an enzyme that degrades basal membrane and its activity is controlled by the tissue inhibitor of metalloproteinase. Methods: Mononuclear cells were obtained from ex-vivo labeling sub-populations of monocytes and MMP-9, and the
frequency was determined by flow cytometry. Culture was performed during 72 hours, stimulating the cells with SLA, levels
of MMP-9 and TIMP-1 in the supernatants were determined by ELISA.
Results: We observed that cells from CL lesions secrete high amounts of MMP-9 when compared to healthy subjects.
Although MMP-9 was produced by monocytes, non-classical ones were the main source of this enzyme. We also observed
that TNF produced in high level during CL contributes to MMP-9 production.
Conclusions: These observations emphasize the role of monocytes, TNF and MMP-9 in the pathogenesis of L. braziliensis
infection. | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, Brazil | pt_BR |
Affilliation | University of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
Affilliation | University of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA | pt_BR |
Affilliation | University of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, Brazil | pt_BR |
Affilliation | Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, Brazil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Subject | Leishmaniasis, Cutaneous | pt_BR |
Subject | Monocytes | pt_BR |
Subject | Tumor Necrosis Factor-alpha | pt_BR |
Subject | Leukocytes, Mononuclear | pt_BR |
Subject | Matrix Metalloproteinase 9 | pt_BR |
Subject | Humans | pt_BR |