Author | Andrade, Dafne Carvalho | |
Author | Borges, Igor Carmo | |
Author | Bouzas, Maiara Lanna Souza Bacelar | |
Author | Oliveira, Juliana R | |
Author | Fukutani, Kiyoshi Ferreira | |
Author | Queiroz, Artur Trancoso Lopo de | |
Author | Oliveira, Camila Indiani de | |
Author | Barral, Aldina Maria Prado | |
Author | Van Weyenbergh, Johan Jozef Rosa Maria | |
Author | Carvalho, Cristiana Maria Costa Nascimento de | |
Access date | 2018-02-06T17:41:08Z | |
Available date | 2018-02-06T17:41:08Z | |
Document date | 2017 | |
Citation | ANDRADE, D. C. et al. 10-valent pneumococcal conjugate vaccine (PCV10) decreases metabolic activity but not nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae. Vaccine, 2017. | pt_BR |
ISSN | 0264-410X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/24744 | |
Sponsorship | Bahia State Agency for Research Funding (FAPESB) (grants APP0045/2009, PNX 0019/2009, PET 007/2012), in Brazil; and FWO (grant G0D6817N) in Belgium. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Polissacarídeos capsulares | pt_BR |
Subject in Portuguese | Metabolismo | pt_BR |
Subject in Portuguese | Colonização nasofaríngea | pt_BR |
Subject in Portuguese | Proteína D | pt_BR |
Title | 10-valent pneumococcal conjugate vaccine (PCV10) decreases metabolic activity but not nasopharyngeal carriage of Streptococcus pneumoniae and Haemophilus influenzae | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.vaccine.2017.06.048 | |
Abstract | The effect of pneumococcal vaccination is widely variable when measured by nasopharyngeal carriage of vaccine and non-vaccine targets. The aim of this study was to compare the carriage rates and metabolic activity of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae and Moraxella catarrhalis among children who were or were not vaccinated with PCV10. Methods: We included children with acute respiratory infection aged 6–23 months from a cross-sectional
study (CHIADO-IVAS). Nasopharyngeal aspirates were collected and respiratory pathogens were quantified
by nCounter digital transcriptomics (Nanostring) and metagenomic sequencing of 16S ribosomal RNA
(Illumina). The metabolic rate was calculated by the ratio between RNA transcripts and 16S DNA reads.
Results: Out of the 80 patients in this study, 53 were vaccinated with PCV10 and 27 were unvaccinated.
There was no difference in nasopharyngeal carriage rates of S. pneumoniae, S. aureus, H. influenzae or M. catarrhalis
by either transcriptomic analysis or 16S metagenomics. However, unvaccinated children presented
a higher metabolic rate for S. pneumoniae compared to PCV10-vaccinated children (Median [25–75th percentiles]:
126 [22.75–218.41] vs. 0[0–47.83], p = 0.004). Furthermore, unvaccinated children presented a
positive correlation between mRNA counts and 16S DNA reads for S. pneumoniae (r = 0.707; p < 0.001)
and H. influenzae (r = 0.525; p = 0.005), in contrast to vaccinated children. No such effect was observed
for S. aureus and M. catarrhalis.
Conclusions: Vaccination by PCV10 exerts a pathogen-specific effect on pneumococcal metabolic rate.
Pathogen RNA/DNA ratio might represent a more sensitive readout for vaccine follow-up, as compared to
nasopharyngeal carriage | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institutos Nacionais de Ciencia e Tecnologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Institutos Nacionais de Ciencia e Tecnologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium | pt_BR |
Affilliation | Federal University of Bahia School of Medicin. Programme in Health Sciences. Salvador, BA, Brazil / Federal University of Bahia School of Medicine. Department of Pediatrics. Salvador, BA, Brazil | pt_BR |
Subject | Capsular polysaccharides | pt_BR |
Subject | Metabolism | pt_BR |
Subject | Nasopharyngeal colonization | pt_BR |
Subject | Protein D | pt_BR |