Author | Flores, Geane Lopes | |
Author | Cruz, Helena Medina | |
Author | Marques, Vanessa Alves | |
Author | Nogueira, Cristiane Alves Villela | |
Author | Potsch, Denise Vigo | |
Author | May, Silvia Beatriz | |
Author | Mello, Carlos Eduardo | |
Author | Pires, Marcia Maria Amendola | |
Author | Pilotto, José Henrique | |
Author | Flores, Priscila Pollo | |
Author | Esberard, Eliane Bordalo Cathalá | |
Author | Ivantes, Claudia | |
Author | Ximenez, Lia Laura Lewis- | |
Author | Lampe, Elisabeth | |
Author | Villar, Livia Melo | |
Access date | 2017-11-30T10:33:57Z | |
Available date | 2017-11-30T10:33:57Z | |
Document date | 2017 | |
Citation | FLORES, Geane Lopes; et al. Performance of ANTI-HCV testing in dried blood spots and saliva according to HIV status. Journal of Medical Virology, v.89, p.1435-1441, 2017. | pt_BR |
ISSN | 0146-6615 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23457 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Vírus da Hepatite C | pt_BR |
Subject in Portuguese | Técnicas Imunoenzimáticas | pt_BR |
Subject in Portuguese | HIV | pt_BR |
Subject in Portuguese | Saliva | pt_BR |
Subject in Portuguese | Mancha de sangue seco | pt_BR |
Title | Performance of ANTI-HCV testing in dried blood spots and saliva according to HIV status | pt_BR |
Type | Article | pt_BR |
DOI | 10.1002/jmv.24777 | |
Abstract | The use of saliva and dried blood spots (DBS) could increase access to HCV diagnosis for high-risk populations, such as HIV-infected individuals, but the performance of these assays has not been well established in this group. This study aims to evaluate HIV status, particularly TCD4+ cell count and viral load, in the performance of anti-HCV testing using DBS and saliva. A total of 961 individuals classified as HCV+, HIV+, or HIV/HCV+, as well as negative controls, donated serum, DBS, and saliva samples for anti-HCV testing using a commercial enzyme immunoassay. Sample volume was modified for DBS and saliva, and an ROC curve was used for cut-off determination in saliva. Anti-HCV sensitivities were greater than 93% using DBS and saliva in the HCV+ group, while they were 83.3% and 95.6% for HCV/HIV+ individuals for DBS and saliva assays, respectively. Specificity varied from 91.7% to 100% using saliva and DBS in HIV monoinfected and control subjects. When only anti-HCV/HCV RNA+ serum samples, that is, true positives, were considered, the sensitivities were 98.3% and 100% for DBS and saliva, respectively, in the HCV+ group and 91.6% and 94.8% for DBS and saliva, respectively, in the HIV/HCV+ group. High absorbance values were observed among those presenting with HCV RNA in serum and low HIV viral load (less than 50 copies/mL). In conclusion, DBS and saliva samples could be used for anti-HCV detection, particularly to identify active HCV cases, but low sensitivity was observed for anti-HCV testing using DBS in the HIV/HCV+ group. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffrée Guinle. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffrée Guinle. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil / Hospital Geral de Nova Iguaçu. Nova Iguaçu, RJ, Brasil. | pt_BR |
Affilliation | Universidade de Brasília. Brasília, DF, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Hospital Universitário Antonio Pedro. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Centro de Orientação e Aconselhamento. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | dried blood spot | pt_BR |
Subject | enzyme immunoassay | pt_BR |
Subject | hepatitis C virus | pt_BR |
Subject | human immunodeficiency virus | pt_BR |
Subject | saliva | pt_BR |
e-ISSN | 1096-9071 | |
Embargo date | 2030-01-01 | |