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SIX RNA VIRUSES AND FORTY-ONE HOSTS: VIRAL SMALL RNAS AND MODULATION OF SMALL RNA REPERTOIRES IN VERTEBRATE AND INVERTEBRATE SYSTEMS
Sistemas vertebrados
Sistemas invertebrados
Hospedeiros
Repertórios de RNA pequenos
Author
Parameswaran, Poornima
Sklan, Ella
Wilkins, Courtney
Burgon, Trever
Samuel, Melanie A.
Lu, Rui
Ansel, K. Mark
Heissmeyer, Vigo
Einav, Shirit
Jackson, William
Doukas, Tammy
Paranjape, Suman
Polacek, Charlotta
Santos, Flávia Barreto dos
Jalili, Roxana
Babrzadeh, Farbod
Gharizadeh, Baback
Grimm, Dirk
Kay, Mark
Koike, Satoshi
Sarnow, Peter
Ronaghi, Mostafa
Ding, Shou-Wei
Harris, Eva
Chow, Marie
Diamond, Michael S.
Kirkegaard, Karla
Glenn, Jeffrey S.
Fire, Andrew Z.
Sklan, Ella
Wilkins, Courtney
Burgon, Trever
Samuel, Melanie A.
Lu, Rui
Ansel, K. Mark
Heissmeyer, Vigo
Einav, Shirit
Jackson, William
Doukas, Tammy
Paranjape, Suman
Polacek, Charlotta
Santos, Flávia Barreto dos
Jalili, Roxana
Babrzadeh, Farbod
Gharizadeh, Baback
Grimm, Dirk
Kay, Mark
Koike, Satoshi
Sarnow, Peter
Ronaghi, Mostafa
Ding, Shou-Wei
Harris, Eva
Chow, Marie
Diamond, Michael S.
Kirkegaard, Karla
Glenn, Jeffrey S.
Fire, Andrew Z.
Affilliation
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA / Tel-Aviv University. Departmento of Clinical Microbiology and Immunology. Tamat-Aviv, Tel-Aviv, Israel.
University of Arkansas for Medical Sciences. Department of Microbiology & Immunology. Little Rock, Arkansas, USA / University of Washington School of Medicine. Department of Immunology. Seatke, Washington, USA,
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / Sg2, Evanston, IL, USA.
Washington University School of Medicine. Washington University School of Medicine. St. Louis, MIssouri, USA / Harvard University. Cambridge, MA, USA.
University of California at Riverside. Department of Plant Pathology & Microbiology. Riverside, CA, USA / Louisiania State University. Department of Biological Sciences. Baton Rouge, Louisiania, USA.
University of California at San Francisco. Strategic Asthma Basic Research Center and the Department of Microbiology & Immunology. San Francisco, CA, USA.
Institute of Molecular Immunology. Helmholtz Center Munich. German Research Center for Environmental Health. Munich, Germany.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA..
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / Medical College of Wisonsin. Center for Biopreparedness and Infectious Disease. Departmento of Microbiology and Molecular Genetics. Milwaukee, WI, USA.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / National Veterinary Institute. Lindholm, Denmark.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA / American Association for the Advancement of Sciences. Science and Technology Policy Fellowship Program. Washington, DC, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA / University of heidelberg. Dept. of Virology. Heidelber, Germany.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
Stanford University School of Medicine. Departments of Pediatrics & Genetics. Stanford, CA, USA.
Stanford University School of Medicine. Departments of Pediatrics & Genetics. Stanford, CA, USA.
Tokyo Metropolitan Institute of Medical Science. Tokyo Metropolitan Organization for Medical Research. Tokio, Japan / Illumina, San Diego, California, United States of America.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
University of California at Riverside. Department of Plant Pathology & Microbiology. Riverside, CA, USA.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
University of Arkansas for Medical Sciences. Department of Microbiology & Immunology. Little Rock, Arkansas, USA.
Washington University School of Medicine. Departments of Medicine, Molecular Microbiology, Pathology & Immunology. St. Louis, Missouri, USA.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA..
Stanford University School of Medicine. Departments of Pathology & Genetics. Stanford, CA, USA.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA / Tel-Aviv University. Departmento of Clinical Microbiology and Immunology. Tamat-Aviv, Tel-Aviv, Israel.
University of Arkansas for Medical Sciences. Department of Microbiology & Immunology. Little Rock, Arkansas, USA / University of Washington School of Medicine. Department of Immunology. Seatke, Washington, USA,
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / Sg2, Evanston, IL, USA.
Washington University School of Medicine. Washington University School of Medicine. St. Louis, MIssouri, USA / Harvard University. Cambridge, MA, USA.
University of California at Riverside. Department of Plant Pathology & Microbiology. Riverside, CA, USA / Louisiania State University. Department of Biological Sciences. Baton Rouge, Louisiania, USA.
University of California at San Francisco. Strategic Asthma Basic Research Center and the Department of Microbiology & Immunology. San Francisco, CA, USA.
Institute of Molecular Immunology. Helmholtz Center Munich. German Research Center for Environmental Health. Munich, Germany.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA..
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / Medical College of Wisonsin. Center for Biopreparedness and Infectious Disease. Departmento of Microbiology and Molecular Genetics. Milwaukee, WI, USA.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA / National Veterinary Institute. Lindholm, Denmark.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA / American Association for the Advancement of Sciences. Science and Technology Policy Fellowship Program. Washington, DC, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA / University of heidelberg. Dept. of Virology. Heidelber, Germany.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
Stanford University School of Medicine. Departments of Pediatrics & Genetics. Stanford, CA, USA.
Stanford University School of Medicine. Departments of Pediatrics & Genetics. Stanford, CA, USA.
Tokyo Metropolitan Institute of Medical Science. Tokyo Metropolitan Organization for Medical Research. Tokio, Japan / Illumina, San Diego, California, United States of America.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA.
Stanford University School of Medicine. Stanford Genome Technology Center. Stanford, CA, USA.
University of California at Riverside. Department of Plant Pathology & Microbiology. Riverside, CA, USA.
University of California at Berkeley. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.
University of Arkansas for Medical Sciences. Department of Microbiology & Immunology. Little Rock, Arkansas, USA.
Washington University School of Medicine. Departments of Medicine, Molecular Microbiology, Pathology & Immunology. St. Louis, Missouri, USA.
Stanford University School of Medicine. Department of Microbiology & Immunology. Stanford, CA, USA.
Stanford University School of Medicine. Department of Gastroenterology & Hepatology. Stanford, CA, USA..
Stanford University School of Medicine. Departments of Pathology & Genetics. Stanford, CA, USA.
Abstract
We have used multiplexed high-throughput sequencing to characterize changes in small RNA populations that occur during viral infection in animal cells. Small RNA-based mechanisms such as RNA interference (RNAi) have been shown in plant and invertebrate systems to play a key role in host responses to viral infection. Although homologs of the key RNAi effector pathways are present in mammalian cells, and can launch an RNAi-mediated degradation of experimentally targeted mRNAs, any role for such responses in mammalian host-virus interactions remains to be characterized. Six different viruses were examined in 41 experimentally susceptible and resistant host systems. We identified virus-derived small RNAs (vsRNAs) from all six viruses, with total abundance varying from "vanishingly rare" (less than 0.1% of cellular small RNA) to highly abundant (comparable to abundant micro-RNAs "miRNAs"). In addition to the appearance of vsRNAs during infection, we saw a number of specific changes in host miRNA profiles. For several infection models investigated in more detail, the RNAi and Interferon pathways modulated the abundance of vsRNAs. We also found evidence for populations of vsRNAs that exist as duplexed siRNAs with zero to three nucleotide 3' overhangs. Using populations of cells carrying a Hepatitis C replicon, we observed strand-selective loading of siRNAs onto Argonaute complexes. These experiments define vsRNAs as one possible component of the interplay between animal viruses and their hosts.
Keywords in Portuguese
Vírus de RNASistemas vertebrados
Sistemas invertebrados
Hospedeiros
Repertórios de RNA pequenos
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