Author | Santos, Fred Luciano Neves | |
Author | Celedon, Paola Alejandra Fiorani | |
Author | Zanchin, Nilson Ivo Tonin | |
Author | Leitolis, Amanda | |
Author | Crestani, Sandra | |
Author | Foti, Leonardo | |
Author | Souza, Wayner Vieira de | |
Author | Gomes, Yara de Miranda | |
Author | Krieger, Marco Aurélio | |
Access date | 2017-09-06T19:44:57Z | |
Available date | 2017-09-06T19:44:57Z | |
Document date | 2017 | |
Citation | SANTOS, Fred Luciano Neves et al. Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. Journal of Clinical Microbiology, v. 55, n. 10, p. 2934-2945, 2017. | pt_BR |
ISSN | 1098-660X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20936 | |
Language | por | pt_BR |
Publisher | American Society For Microbiology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/JCM.00851-17 | |
Abstract | Diagnosing chronic Chagas disease (CD) requires antibody--antigen detection methods, traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. Previously, 4 recombinant chimeric proteins from Instituto de Biologia Molecular do Paraná (IBMP-8.1 to -8.4) comprising immuno-dominant regions of diverse Trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. Considering that next-generation platforms offer improved CD diagnostic accuracy with different T. cruzi-specific recombinant antigens, we assessed the performance of these chimeras in liquid microarrays (LMAs). The chimeric proteins were expressed in Escherichia coli and purified by chromatography. Sera from 653 chagasic and 680 healthy individuals were used to assess the performance of these chimeras in detecting specific anti-T. cruzi antibodies. Accuracies ranged from 98.1--99.3%, and diagnostic odds ratio values were 3,548 for IBMP-8.3, 4,826 for IBMP-8.1, 7,882 for IBMP-8.2, and 25,000 for IBMP-8.4. A separate sera bank (851 samples) was employed to assess cross-reactivity with other tropical diseases. Leishmania spp., a pathogen with high genome sequence similar to T. cruzi, showed cross-reactivity rates ranging from 0--2.17%. Inconclusive results were negligible (0--0.71%). Bland--Altman and Deming regression analysis based on 200 randomly selected CD-positive and -negative samples demonstrated interchangeability with respect to CD diagnostic performance in both singleplex and multiplex assays. Our results suggested that these chimeras can potentially replace antigens currently used in commercially available assay kits. Moreover, the use of a multiplex platform, like LMA assays employing 2 or more IBMP antigens, would abrogate the need for 2 different testing techniques when diagnosing CD. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Chagas Disease | pt_BR |
Subject | Mutant Chimeric Proteins | pt_BR |
Subject | Antigens | pt_BR |
Subject in Spanish | Enfermedad de Chagas | pt_BR |
Subject in Spanish | Proteínas Mutantes Quiméricas | pt_BR |
Subject in Spanish | Antígenos | pt_BR |
DeCS | Doença de Chagas | pt_BR |
DeCS | Proteínas Mutantes Quiméricas | pt_BR |
DeCS | Antígenos | pt_BR |