Author | Trevisan-Silva, Dilza | |
Author | Bednaski, Aline V. | |
Author | Fischer, Juliana de Saldanha da Gama | |
Author | Veiga, Silvio S. | |
Author | Bandeira, Nuno | |
Author | Guthals, Adrian | |
Author | Marchini, Fabrício Klerynton | |
Author | Leprevost, Felipe da Veiga | |
Author | Barbosa, Valmir Carneiro | |
Author | Senff-Ribeiro, Andrea | |
Author | Carvalho, Paulo Costa | |
Access date | 2017-09-06T18:49:14Z | |
Available date | 2017-09-06T18:49:14Z | |
Document date | 2017 | |
Citation | TREVISAN-SILVA, Dilza et al. Data Descriptor: A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom. Scientific Data, v. 4, p.1-7, 2017. | pt_BR |
ISSN | 2052-4463 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20935 | |
Language | por | pt_BR |
Publisher | Nature Publishing Group | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Aranha Marrom Reclusa | pt_BR |
Title | A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/sdata.2017.90 | |
Abstract | Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Center for Computational Mass Spectrometry. University of California. San Diego, USA. | pt_BR |
Affilliation | Center for Computational Mass Spectrometry. University of California. San Diego, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas RPT02H. Curitiba, PR, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Federal University of Rio de Janeiro. COPPE. Systems Engineering and Computer Science Program. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil. | pt_BR |
Subject | Brown Recluse Spider | pt_BR |
Subject | Spider Venoms | pt_BR |
Subject | Sequence Analysis | pt_BR |
Subject in Spanish | Araña Reclusa Parda | pt_BR |
Subject in Spanish | Venenos de Araña | pt_BR |
Subject in Spanish | Análisis de Secuencia de Proteína | pt_BR |
DeCS | Venenos de Aranha | pt_BR |
DeCS | Análise de Sequência de Proteína | pt_BR |