Author | Shigunov, Patrícia | |
Author | Dallagiovanna, Bruno | |
Access date | 2017-08-18T13:29:54Z | |
Available date | 2017-08-18T13:29:54Z | |
Document date | 2015 | |
Citation | SHIGUNOV P.; DALLAGIOVANNA B. Stem cell ribonomics: RNA-binding proteins and gene networks in Stem cell differentiation. Frontiers in Molecular Biosciences, v. 2, n. 74, p. 1-7, 2015. | pt_BR |
ISSN | 2296-889X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20676 | |
Language | por | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Diferenciação Celular | pt_BR |
Title | Stem cell ribonomics: RNA-binding proteins and gene networks in Stem cell differentiation | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fmolb.2015.00074 | pt_BR |
Abstract | Stem cells are undifferentiated cells with the ability to self-renew and the potential to differentiate into all body cell types. Stem cells follow a developmental genetic program and are able to respond to alterations in the environment through various signaling pathways. The mechanisms that control these processes involve the activation of transcription followed by a series of post-transcriptional events. These post-transcriptional steps are mediated by the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs creating a regulatory gene network. Characterizing these RNA-protein networks is essential to understanding the regulatory mechanisms underlying the control of stem cell fate. Ribonomics is the combination of classical biochemical purification protocols with the high-through put identification of transcripts applied to the functional characterization of RNA-protein complexes. Here, we describe the different approaches that can be used in a ribonomic approach and how they have contributed to understanding the function of several RBPs with central roles in stem cell biology. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Subject | Ribonomics | pt_BR |
Subject | RNA-Binding Proteins | pt_BR |
Subject | Stem Cells | pt_BR |
Subject | Gene Regulatory Networks | pt_BR |
Subject | Cell Differentiation | pt_BR |
Subject in Spanish | Proteínas de Unión al ARN | pt_BR |
Subject in Spanish | Células Madre | pt_BR |
Subject in Spanish | Redes Reguladoras de Genes | pt_BR |
Subject in Spanish | Diferenciación Celular | pt_BR |
DeCS | Proteínas de Ligação a RNA | pt_BR |
DeCS | Células-Tronco | pt_BR |
DeCS | Redes Reguladoras de Genes | pt_BR |