Author | Dória, Grace Anne Azevedo | |
Author | Menezes, Paula dos Passos | |
Author | Lima, Bruno dos Santos | |
Author | Vasconcelos, Bruno S | |
Author | Silva, Francilene Amaral da | |
Author | Henriques, Raíssa Melo | |
Author | Melo, Marcélia Garcez Dória de | |
Author | Alves, Ângela Valéria Farias | |
Author | Moraes, Manoel O | |
Author | Pessoa, Cláudia do Ó | |
Author | Carvalho, Adriana Andrade | |
Author | Prata, Ana Paula do Nascimento | |
Author | Albuquerque Junior, Ricardo Luiz Cavalcanti de | |
Author | Verde, Isabel Bezerra Lima | |
Author | Quintans Júnior, Lucindo José | |
Author | Bezerra, Daniel Pereira | |
Author | Nogueira, Paulo Cesar de Lima | |
Author | Araujo, Adriano Antunes de Souza | |
Access date | 2017-08-17T14:10:19Z | |
Available date | 2017-08-17T14:10:19Z | |
Document date | 2016 | |
Citation | DORIA, G. A. A. et al. In vivo antitumor effect, induction of apoptosis and safety of Remirea maritima Aubl. (Cyperaceae) extracts. Phytomedicine, v. 23, p. 914–922, 2016. | pt_BR |
ISSN | 0944-7113 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20659 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Remirea maritima | pt_BR |
Subject in Portuguese | Antitumor | pt_BR |
Subject in Portuguese | Sarcoma 180 | pt_BR |
Subject in Portuguese | Apoptose | pt_BR |
Subject in Portuguese | Toxicidade | pt_BR |
Subject in Portuguese | Tunel | pt_BR |
Title | In vivo antitumor effect, induction of apoptosis and safety of Remirea maritima Aubl. (Cyperaceae) extracts | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.phymed.2016.05.001 | |
Abstract | Remirea maritima has been widely used in the treatment of diarrhea, kidney disease, and high fever and for therapeutic purposes, such as an analgesic and anti-inflammatory. However, few scientific research studies on its medicinal properties have been reported. Purpose: The present study aimed to investigate the anticancer potential of aqueous extract (AE), 40% hydroalcoholic extracts (40HA) and 70% (70HA) from R. maritima in experimental models and to identify its phytochemical compounds. Methods: The chemical composition of AE, 40HA and 70HA was assessed by HPLC-DAD and ESI-IT-MS/MS . In vitro activity was determined on cultured tumor cell, NCI-H385N (Broncho-alveolar carcinoma), OVCAR- 8 (Ovarian carcinoma) and PC-3 M (prostate carcinoma) by the MTT assay, and the in vivo antitumor activity was assessed in Sarcoma 180-bearing mice. Toxicological parameters were also evaluated as well as the humoral immune response. Results: Among the aqueous and hydroalcoholic extracts of R. maritima , only 40HA showed in vitro bi- ological effect potential, presenting IC 50 values of 27.08, 46.62 and > 50 μg/ml for OVCAR-8, NCI-H385M and PC-3 M cells lines, respectively. Regarding chemical composition, a mixture of isovitexin-2 - O - β-D- glucopyranoside, vitexin-2 - O - β-D-glucopyranoside, luteolin-7- O -glucuronide and 1- O -(E)-caffeoyl- β-D- glucose were identified as the major phytochemical compounds of the extracts. In the in vivo study, the tumor inhibition rates were 57.16–62.57% at doses of 25 mg/kg and 50 mg/kg, respectively, and the tu- mor morphology presented increasing numbers of apoptotic cells. Additionally, 40HA also demonstrated significantly increased of OVA-specific total Ig. Conclusions: 40HA exhibited in vitro and in vivo anticancer properties without substantial toxicity that could be associated with its immunostimulating properties. | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil | pt_BR |
Affilliation | Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil | pt_BR |
Affilliation | Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brazil | pt_BR |
Affilliation | Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil | pt_BR |
Subject | Remirea maritima | pt_BR |
Subject | Antitumor | pt_BR |
Subject | Sarcoma 180 | pt_BR |
Subject | Apoptosis | pt_BR |
Subject | Toxicity | pt_BR |
Subject | Tunel | pt_BR |