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2025-01-01
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RESPONSES OF MICROBIAL COMMUNITY FROM TROPICAL PRISTINE COASTAL SOIL TO CRUDE OIL CONTAMINATION.
Beta-diversity
Crude oil
Metagenome
Next generation sequencing
Taxonomy comparison
Affilliation
Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil/Rothamsted Research. AgroEcology Department. Harpenden, Hertfordshire, United Kingdom
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil
Rothamsted Research. AgroEcology Department. Harpenden, Hertfordshire, United Kingdom
Rothamsted Research. AgroEcology Department. Harpenden, Hertfordshire, United Kingdom
Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brazil
Rothamsted Research. AgroEcology Department. Harpenden, Hertfordshire, United Kingdom
Rothamsted Research. AgroEcology Department. Harpenden, Hertfordshire, United Kingdom
Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brazil
Abstract
Brazilian offshore crude oil exploration has increased after the discovery of new reservoirs in the region known as pré-sal, in a depth of 7.000 m under the water surface. Oceanic islands near these areas represent sensitive environments, where changes in microbial communities due oil contamination could stand for the loss of metabolic functions, with catastrophic effects to the soil services provided from these locations. This work aimed to evaluate the effect of petroleum contamination on microbial community shifts (Archaea, Bacteria and Fungi) from Trindade Island coastal soils. Microcosms were assembled and divided in two treatments, control and contaminated (weathered crude oil at the concentration of 30 g kg(-1)), in triplicate. Soils were incubated for 38 days, with CO2 measurements every four hours. After incubation, the total DNA was extracted, purified and submitted for target sequencing of 16S rDNA, for Bacteria and Archaea domains and Fungal ITS1 region, using the Illumina MiSeq platform. Three days after contamination, the CO2 emission rate peaked at more than 20 × the control and the emissions remained higher during the whole incubation period. Microbial alpha-diversity was reduced for contaminated-samples. Fungal relative abundance of contaminated samples was reduced to almost 40% of the total observed species. Taxonomy comparisons showed rise of the Actinobacteria phylum, shifts in several Proteobacteria classes and reduction of the Archaea class Nitrososphaerales. This is the first effort in acquiring knowledge concerning the effect of crude oil contamination in soils of a Brazilian oceanic island. This information is important to guide any future bioremediation strategy that can be required.
Keywords in Portuguese
contaminaçãoKeywords
Alpha-diversityBeta-diversity
Crude oil
Metagenome
Next generation sequencing
Taxonomy comparison
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