Author | Coutinho, Mariana Loner | |
Author | Vasconcellos, Flavia Aleixo | |
Author | Fernandes, C. P. H. | |
Author | Seyffert, Núbia | |
Author | Seixas, Fabiana Kommling | |
Author | Ko, Albert Icksang | |
Author | Dellagostin, Odir Antonio | |
Author | Aleixo, José Antonio Guimarães | |
Access date | 2017-07-18T12:20:42Z | |
Available date | 2017-07-18T12:20:42Z | |
Document date | 2007 | |
Citation | COUTINHO, Mariana Loner et al. Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal of Immunoassay and Immunochemistry, v. 28, p. 279-288, 2007. | pt_BR |
ISSN | 1532-1819 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20125 | |
Sponsorship | Fundação Oswaldo Cruz (09224-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (477609/2004-0,301920/2006-0 and 504227/2006-0) and National Institutes of Health, USA (AI034431 and
TW00919). | pt_BR |
Language | eng | pt_BR |
Publisher | Taylor & Francis | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leptospira | pt_BR |
Subject in Portuguese | Antígenos | pt_BR |
Subject in Portuguese | Afinidade de anticorpos | pt_BR |
Subject in Portuguese | Mapeamento de epítopos | pt_BR |
Subject in Portuguese | ELISA | pt_BR |
Subject in Portuguese | Western Blot | pt_BR |
Title | Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests | pt_BR |
Type | Article | pt_BR |
DOI | 10.1080/15321810701454870 | |
Abstract | LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection. | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RGS, Brazil. | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil. | pt_BR |
Affilliation | Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil. | pt_BR |
Subject | Leptospira | pt_BR |
Subject | Recombinant antigens, | pt_BR |
Subject | Antibody affinity | pt_BR |
Subject | Epitope mapping | pt_BR |
Subject | ELISA | pt_BR |
Subject | Western blotting | pt_BR |