Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18978
Title: Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
Authors: Saldanha, Maíra Garcia
Queiroz, Adriano
Machado, Paulo Roberto Lima
Carvalho, Lucas Pedreira de
Scott, Phillip
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
Affilliation: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Cutaneous leishmaniasis (CL), characterized by an ulcerated lesion, is the most common clinical form of human leishmaniasis. Before the ulcer develops, patients infected with Leishmania (Viannia) braziliensis present a small papule at the site of the sandfly bite, referred to as early cutaneous leishmaniasis (E-CL). Two to four weeks later the typical ulcer develops, which is considered here as late CL (L-CL). Although there is a great deal known about T-cell responses in patients with L-CL, there is little information about the in situ inflammatory response in E-CL. Histological sections of skin biopsies from 15 E-CL and 28 L-CL patients were stained by hematoxilin and eosin to measure the area infiltrated by cells, as well as tissue necrosis. Leishmania braziliensis amastigotes, CD4(+), CD8(+), CD20(+), and CD68(+) cells were identified and quantified by immunohistochemistry. The number of amastigotes in E-CL was higher than in L-CL, and the inflammation area was larger in classical ulcers than in E-CL. There was no relationship between the number of parasites and magnitude of the inflammation area, or with the lesion size. However, there was a direct correlation between the number of macrophages and the lesion size in E-CL, and between the number of macrophages and necrotic area throughout the course of the disease. These positive correlations suggest that macrophages are directly involved in the pathology of L. braziliensis-induced lesions.
Keywords: Cutaneous Leishmaniasis
Leishmania (Viannia) braziliensis
Histopathology
Biopsies
Cutaneous ulcer
Macrophages
keywords: Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biopsia
Úlcera cutânea
Macrófagos
DeCS: Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biópsia
Úlcera cutânea
Macrófagos
Issue Date: 2017
Publisher: American Society of Tropical Medicine and Hygiene
Citation: SALDANHA, M. G. et al. Characterization of the histopathologic features in patients in the early and late phases of Cutaneous Leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 96, n. 3, p. 645–652, 2017.
DOI: 10.4269/ajtmh.16-0539
ISSN: 0002-9637
Copyright: restricted access
Appears in Collections:IGM - Artigos de Periódicos

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