Author | Davis, Richard E | |
Author | Sharma, Smriti | |
Author | Conceição, Jacilara Alexandrino | |
Author | Carneiro, Pedro Paulo Oliveira | |
Author | Novais, Fernanda Oliveira | |
Author | Scott, Phillip | |
Author | Sundar, Shyam | |
Author | Bacellar, Maria Olívia Amado Ramos | |
Author | Carvalho Filho, Edgar Marcelino | |
Author | Wilson, Mary E | |
Access date | 2017-05-08T16:47:48Z | |
Available date | 2017-05-08T16:47:48Z | |
Document date | 2017 | |
Citation | DAVIS, R. E. et al. Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis. Journal of Leukocyte Biology, v. 101, p. 739–749, 2017. | pt_BR |
ISSN | 0741-5400 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18783 | |
Sponsorship | Tropical Medicine Research Center from the U.S. National Institutes of Health (Grant P50 AI-30639 to O.B., E.M.C., and M.E.W. and Grants R01 AI076233 and R01 AI045540), a Merit Review grant from the Department of Veterans’ Affairs (Grant 5I01BX001983), and a Major Project Award and Travel grant from the Stanley Foundation/University of Iowa
Iowa. | pt_BR |
Language | eng | pt_BR |
Publisher | Society for Leukocyte Biology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Neutrófilo | pt_BR |
Subject in Portuguese | Granulócito | pt_BR |
Title | Phenotypic and functional characteristics of HLA-DR(+) neutrophils in Brazilians with cutaneous leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1189/jlb.4A0915-442RR | |
Abstract | The protozoan Leishmania braziliensis causes cutaneous leishmaniasis (CL) in endemic regions. In murine models, neutrophils (PMNs) are recruited to the site of infection soon after parasite inoculation. However, the roles of neutrophils during chronic infection and in human disease remain undefined. We hypothesized that neutrophils help maintain a systemic inflammatory state in subjects with CL. Lesion biopsies from all patients with CL tested contained neutrophils expressing HLA-DR, a molecule thought to be restricted to professional antigen-presenting cells. Although CL is a localized disease, a subset of patients with CL also had circulating neutrophils expressing HLA-DR and the costimulatory molecules CD80, CD86, and CD40. PMNs isolated from a low-density leukocyte blood fraction (LD-PMNs) contained a higher percentage of HLA-DR(+) PMNs than did normal-density PMNs. In vitro coculture experiments suggested LD-PMNs do not suppress T cell responses, differentiating them from MDSCs. Flow-sorted HLA-DR(+) PMNs morphologically resembled conventional PMNs, and they exhibited functional properties of PMNs. Compared with conventional PMNs, HLA-DR(+) PMNs showed increased activation, degranulation, DHR123 oxidation, and phagocytic capacity. A few HLA-DR(+) PMNs were observed in healthy subjects, and that proportion could be increased by incubation in either inflammatory cytokines or in plasma from a patient with CL. This was accompanied by an increase in PMN hladrb1 mRNA, suggesting a possible connection between neutrophil "priming" and up-regulation of HLA-DR. These data suggest that PMNs that are primed for activation and that also express surface markers of antigen-presenting cells emerge in the circulation and infected tissue lesions of patients with CL. | pt_BR |
Affilliation | University of Iowa. Departments of Internal Medicine and Microbiology. Iowa City, Iowa, USA | pt_BR |
Affilliation | Banaras Hindu University. Institute of Medical Sciences. Varanasi, Uttar Pradesh, India | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
Affilliation | Banaras Hindu University. Institute of Medical Sciences. Varanasi, Uttar Pradesh, India | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Iowa. Departments of Internal Medicine and Microbiology. Iowa City, Iowa, USA / Iowa City Veterans’ Affairs Medical Center. Research Service. Iowa City, Iowa, USA | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Neutrophil | pt_BR |
Subject | Granulocyte | pt_BR |
DeCS | Leishmania | pt_BR |
DeCS | Neutrófilos | pt_BR |
DeCS | Granulócitos | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |