Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18598
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dc.contributor.authorBencze, Maximilien
dc.contributor.authorNegroni, Elisa
dc.contributor.authorVallese, Denis
dc.contributor.authorYacoub-Youssef, Houda
dc.contributor.authorChaouch, Soraya
dc.contributor.authorWolff, Annie
dc.contributor.authorAamiri, Ahmed
dc.contributor.authorDi Santo, James P.
dc.contributor.authorChazaud, Bénédicte
dc.contributor.authorButler-Browne, Gillian
dc.contributor.authorSavino, Wilson
dc.contributor.authorMouly, Vincent
dc.contributor.authorRiederer, Ingo
dc.date.accessioned2017-04-20T16:04:56Z
dc.date.available2017-04-20T16:04:56Z
dc.date.issued2012
dc.identifier.citationBENCZE, Maximilien; et al. Proinflammatory Macrophages Enhance the Regenerative Capacity of Human Myoblasts by Modifying Their Kinetics of Proliferation and Differentiation. Molecular Therapy, v.20, n.11, p.2168-2179, Nov. 2012.
dc.identifier.issn1525-0016
dc.identifier.urihttp://www.arca.fiocruz.br/handle/icict/18598
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subject.otherMioblastos Humanos
dc.subject.otherCapacidade Regenerativa
dc.subject.otherMacrófagos proinflamatórios
dc.subject.otherCinética
dc.titleProinflammatory macrophages enhance the regenerative capacity of human myoblasts by modifying their kinetics of proliferation and differentiation
dc.typeArticle
dc.identifier.doi10.1038/mt.2012.189
dc.description.abstractenMacrophages have been shown to be essential for muscle repair by delivering trophic cues to growing skeletal muscle precursors and young fibers. Here, we investigated whether human macrophages, either proinflammatory or anti-inflammatory, coinjected with human myoblasts into regenerating muscle of Rag2(-/-) γC(-/-) immunodeficient mice, could modify in vivo the kinetics of proliferation and differentiation of the transplanted human myogenic precursors. Our results clearly show that proinflammatory macrophages improve in vivo the participation of injected myoblasts to host muscle regeneration, extending the window of proliferation, increasing migration, and delaying differentiation. Interestingly, immunostaining of transplanted proinflammatory macrophages at different time points strongly suggests that these cells are able to switch to an anti-inflammatory phenotype in vivo, which then may stimulate differentiation during muscle regeneration. Conceptually, our data provide for the first time in vivo evidence strongly suggesting that proinflammatory macrophages play a supportive role in the regulation of myoblast behavior after transplantation into preinjured muscle, and could thus potentially optimize transplantation of myogenic progenitors in the context of cell therapy.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationINSERM, U1016. Institut Cochin. Paris, France / Université Paris Descartes. Paris, France.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationUniversité d’Agadir. Faculté des Sciences. Départment de Biologie. Agadir, Marocco.
dc.creator.affilliationInnate Immunity Unit. Inserm U668. Institut Pasteur. Paris, France.
dc.creator.affilliationINSERM. U1016. Institut Cochin, Paris, France / Université Paris Descartes. Paris, France.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
dc.creator.affilliationCNRS, UMR7215., Institut de Myologie. Paris, France / Université Pierre et Marie Curie. UM76. Paris, France.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
dc.subject.enproinflammatory macrophages
dc.subject.enHuman Myoblasts
dc.subject.enRegenerative Capacity
dc.subject.enkinetics
dc.identifier.eissn1525-0024
Appears in Collections:IOC - Artigos de Periódicos

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