Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18591
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRiederer, Ingo-
dc.contributor.authorNegroni, Elisa-
dc.contributor.authorBencze, Maximilien-
dc.contributor.authorWolff, Annie-
dc.contributor.authorAamiri, Ahmed-
dc.contributor.authorDi Santo, James P.-
dc.contributor.authorBarbosa, Suse D. Silva-
dc.contributor.authorButler-Browne, Gillian-
dc.contributor.authorSavino, Wilson-
dc.contributor.authorMouly, Vincent-
dc.date.accessioned2017-04-20T13:36:17Z-
dc.date.available2017-04-20T13:36:17Z-
dc.date.issued2012-
dc.identifier.citationRIEDERER, Ingo; et al. Slowing Down Differentiation of Engrafted Human Myoblasts Into Immunodeficient Mice Correlates With Increased Proliferation and Migration. Molecular Therapy, v.20, n.1, p.146-154, jan. 2012.pt_BR
dc.identifier.issn1525-0016pt_BR
dc.identifier.urihttp://www.arca.fiocruz.br/handle/icict/18591-
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestricted accesspt_BR
dc.subject.otherDesaceleração da diferenciaçãopt_BR
dc.subject.otherMioblastos humanos enxertadospt_BR
dc.subject.otherRatos imunideficientespt_BR
dc.subject.otherMigraçãopt_BR
dc.subject.otherProliferaçãopt_BR
dc.titleSlowing down differentiation of engrafted human myoblasts into immunodeficient mice correlates with increased proliferation and migrationpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1038/mt.2011.193-
dc.description.abstractenWe have used a model of xenotransplantation in which human myoblasts were transplanted intramuscularly into immunodeficient Rag2(-/-)γC(-/-) mice, in order to investigate the kinetics of proliferation and differentiation of the transplanted cells. After injection, most of the human myoblasts had already differentiated by day 5. This differentiation correlated with reduction in proliferation and limited migration of the donor cells within the regenerating muscle. These results suggest that the precocious differentiation, already detected at 3 days postinjection, is a limiting factor for both the migration from the injection site and the participation of the donor cells to muscle regeneration. When we stimulated in vivo proliferation of human myoblasts, transplanting them in a serum-containing medium, we observed 5 days post-transplantation a delay of myogenic differentiation and an increase in cell numbers, which colonized a much larger area within the recipient's muscle. Importantly, these myoblasts maintained their ability to differentiate, since we found higher numbers of myofibers seen 1 month postengraftment, as compared to controls. Conceptually, these data suggest that in experimental myoblast transplantation, any intervention upon the donor cells and/or the recipient's microenvironment aimed at enhancing proliferation and migration should be done before differentiation of the implanted cells, e.g., day 3 postengraftment.pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, Francept_BR
dc.creator.affilliationLaboratoire LBCM. Departement de Biologie. Faculté des Sciences. Agadir, Morocco.pt_BR
dc.creator.affilliationInnate Immunity Unit. Department of Immunology. Institut Pasteur. INSERM-U668, Paris, France.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Departamento de Pesquisa Clínica. Rio de Janeiro, RJ, Brasil.pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .pt_BR
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.pt_BR
dc.subject.enImmunodeficient Micept_BR
dc.subject.enEngrafted Human Myoblastspt_BR
dc.subject.enProliferationpt_BR
dc.subject.enMigrationpt_BR
dc.subject.enSlowing Down Differentiationpt_BR
dc.identifier.eissn1525-0024-
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
wilson3_savino_etal_IOC_2012.pdf1.06 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.