Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18591
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dc.contributor.authorRiederer, Ingo
dc.contributor.authorNegroni, Elisa
dc.contributor.authorBencze, Maximilien
dc.contributor.authorWolff, Annie
dc.contributor.authorAamiri, Ahmed
dc.contributor.authorDi Santo, James P.
dc.contributor.authorBarbosa, Suse D. Silva
dc.contributor.authorButler-Browne, Gillian
dc.contributor.authorSavino, Wilson
dc.contributor.authorMouly, Vincent
dc.date.accessioned2017-04-20T13:36:17Z
dc.date.available2017-04-20T13:36:17Z
dc.date.issued2012
dc.identifier.citationRIEDERER, Ingo; et al. Slowing Down Differentiation of Engrafted Human Myoblasts Into Immunodeficient Mice Correlates With Increased Proliferation and Migration. Molecular Therapy, v.20, n.1, p.146-154, jan. 2012.
dc.identifier.issn1525-0016
dc.identifier.urihttp://www.arca.fiocruz.br/handle/icict/18591
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subject.otherDesaceleração da diferenciação
dc.subject.otherMioblastos humanos enxertados
dc.subject.otherRatos imunideficientes
dc.subject.otherMigração
dc.subject.otherProliferação
dc.titleSlowing down differentiation of engrafted human myoblasts into immunodeficient mice correlates with increased proliferation and migration
dc.typeArticle
dc.identifier.doi10.1038/mt.2011.193
dc.description.abstractenWe have used a model of xenotransplantation in which human myoblasts were transplanted intramuscularly into immunodeficient Rag2(-/-)γC(-/-) mice, in order to investigate the kinetics of proliferation and differentiation of the transplanted cells. After injection, most of the human myoblasts had already differentiated by day 5. This differentiation correlated with reduction in proliferation and limited migration of the donor cells within the regenerating muscle. These results suggest that the precocious differentiation, already detected at 3 days postinjection, is a limiting factor for both the migration from the injection site and the participation of the donor cells to muscle regeneration. When we stimulated in vivo proliferation of human myoblasts, transplanting them in a serum-containing medium, we observed 5 days post-transplantation a delay of myogenic differentiation and an increase in cell numbers, which colonized a much larger area within the recipient's muscle. Importantly, these myoblasts maintained their ability to differentiate, since we found higher numbers of myofibers seen 1 month postengraftment, as compared to controls. Conceptually, these data suggest that in experimental myoblast transplantation, any intervention upon the donor cells and/or the recipient's microenvironment aimed at enhancing proliferation and migration should be done before differentiation of the implanted cells, e.g., day 3 postengraftment.
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France
dc.creator.affilliationLaboratoire LBCM. Departement de Biologie. Faculté des Sciences. Agadir, Morocco.
dc.creator.affilliationInnate Immunity Unit. Department of Immunology. Institut Pasteur. INSERM-U668, Paris, France.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Departamento de Pesquisa Clínica. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .
dc.creator.affilliationThérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
dc.subject.enImmunodeficient Mice
dc.subject.enEngrafted Human Myoblasts
dc.subject.enProliferation
dc.subject.enMigration
dc.subject.enSlowing Down Differentiation
dc.identifier.eissn1525-0024
Appears in Collections:IOC - Artigos de Periódicos

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