Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18591
Title: Slowing down differentiation of engrafted human myoblasts into immunodeficient mice correlates with increased proliferation and migration
Authors: Riederer, Ingo
Negroni, Elisa
Bencze, Maximilien
Wolff, Annie
Aamiri, Ahmed
Di Santo, James P.
Barbosa, Suse D. Silva
Butler-Browne, Gillian
Savino, Wilson
Mouly, Vincent
Affilliation: Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France
Laboratoire LBCM. Departement de Biologie. Faculté des Sciences. Agadir, Morocco.
Innate Immunity Unit. Department of Immunology. Institut Pasteur. INSERM-U668, Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Câncer. Departamento de Pesquisa Clínica. Rio de Janeiro, RJ, Brasil.
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. .
Thérapie des maladies du muscle strié/Institut de Myologie UM76. Université Pierre et Marie Curie, INSERM-U974; CNRS-UMR7215. Paris, France.
Abstract: We have used a model of xenotransplantation in which human myoblasts were transplanted intramuscularly into immunodeficient Rag2(-/-)γC(-/-) mice, in order to investigate the kinetics of proliferation and differentiation of the transplanted cells. After injection, most of the human myoblasts had already differentiated by day 5. This differentiation correlated with reduction in proliferation and limited migration of the donor cells within the regenerating muscle. These results suggest that the precocious differentiation, already detected at 3 days postinjection, is a limiting factor for both the migration from the injection site and the participation of the donor cells to muscle regeneration. When we stimulated in vivo proliferation of human myoblasts, transplanting them in a serum-containing medium, we observed 5 days post-transplantation a delay of myogenic differentiation and an increase in cell numbers, which colonized a much larger area within the recipient's muscle. Importantly, these myoblasts maintained their ability to differentiate, since we found higher numbers of myofibers seen 1 month postengraftment, as compared to controls. Conceptually, these data suggest that in experimental myoblast transplantation, any intervention upon the donor cells and/or the recipient's microenvironment aimed at enhancing proliferation and migration should be done before differentiation of the implanted cells, e.g., day 3 postengraftment.
Keywords: Immunodeficient Mice
Engrafted Human Myoblasts
Proliferation
Migration
Slowing Down Differentiation
keywords: Desaceleração da diferenciação
Mioblastos humanos enxertados
Ratos imunideficientes
Migração
Proliferação
Issue Date: 2012
Publisher: Elsevier
Citation: RIEDERER, Ingo; et al. Slowing Down Differentiation of Engrafted Human Myoblasts Into Immunodeficient Mice Correlates With Increased Proliferation and Migration. Molecular Therapy, v.20, n.1, p.146-154, jan. 2012.
DOI: 10.1038/mt.2011.193
ISSN: 1525-0016
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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