Author | Andrade, Warrison A. | |
Author | Agarwal, Sarika | |
Author | Mo, Shunyan | |
Author | Shaffer, Scott A. | |
Author | Dillard, Joseph P. | |
Author | Schmidt, Tobias | |
Author | Hornung, Veit | |
Author | Kurt-Jones, Evelyn A. | |
Author | Golenbock, Douglas T. | |
Access date | 2017-04-12T18:42:07Z | |
Available date | 2017-04-12T18:42:07Z | |
Document date | 2016 | |
Citation | ANDRADE, Warrison A et al. Type I Interferon Induction by Neisseria gonorrhoeae: Dual Requirement of Cyclic GMP-AMP Synthase and Toll-like Receptor 4. Cell Reports, v. 15, n. 11, p. 2438 – 2448, 2016 | pt_BR |
ISSN | 2211-1247 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18431 | |
Language | eng | pt_BR |
Publisher | Cell Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Neisseria gonorrhoeae | pt_BR |
Subject in Portuguese | sistema imune inato | pt_BR |
Title | Type I Interferon Induction by Neisseria gonorrhoeae: Dual Requirement of Cyclic GMP-AMP Synthase and Toll-like Receptor 4 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.celrep.2016.05.030. | |
Abstract | The innate immune system is the first line of defense against Neisseria gonorrhoeae (GC). Exposure of cells to GC lipooligosaccharides induces a strong immune response, leading to type I interferon (IFN) production via TLR4/MD-2. In addition to living freely in the extracellular space, GC can invade the cytoplasm to evade detection and elimination. Double-stranded DNA introduced into the cytosol binds and activates the enzyme cyclic-GMP-AMP synthase (cGAS), which produces 2′3′-cGAMP and triggers STING/TBK-1/IRF3 activation, resulting in type I IFN expression. Here, we reveal a cytosolic response to GC DNA that also contributes to type I IFN induction. We demonstrate that complete IFN-β induction by live GC depends on both cGAS and TLR4. Type I IFN is detrimental to the host, and dysregulation of iron homeostasis genes may explain lower bacteria survival in cGAS−/− and TLR4−/− cells. Collectively, these observations reveal cooperation between TLRs and cGAS in immunity to GC infection. | pt_BR |
Affilliation | University of Massachusetts Medical School. Division of Infectious Diseases. Worcester, MA, USA/University of Massachusetts Medical School. Department of Medicine. Program in Innate Immunity. Worcester, MA, USA | pt_BR |
Affilliation | University of Massachusetts Medical School. Division of Infectious Diseases. Worcester, MA, USA | pt_BR |
Affilliation | University of Massachusetts Medical School. Proteomics and Mass Spectrometry Facility. Worcester, MA, USA/University of Massachusetts Medical School. Department of Biochemistry and Molecular Pharmacology. Worcester, MA, USA | pt_BR |
Affilliation | University of Massachusetts Medical School. Proteomics and Mass Spectrometry Facility. Worcester, MA, USA/University of Massachusetts Medical School. Department of Biochemistry and Molecular Pharmacology. Worcester, MA, USA | pt_BR |
Affilliation | University of Wisconsin-Madison. Department of Medical Microbiology and Immunology. Madison, WI, USA | pt_BR |
Affilliation | Universitätsklinikum Bonn. Institute of Molecular Medicine. Bonn,Germany | pt_BR |
Affilliation | Universitätsklinikum Bonn. Institute of Molecular Medicine. Bonn,Germany Katherine A. Fitzgerald/University of Massachusetts Medical School. Division of Infectious Diseases. Worcester, MA, USA/University of Massachusetts Medical School. Department of Medicine. Program in Innate Immunity. Worcester, MA, USA/Norwegian University of Science and Technology. Centre of Molecular Inflammation Research and Department of Cancer Research and Molecular Medicine. Trondheim, Norway | pt_BR |
Affilliation | University of Massachusetts Medical School. Division of Infectious Diseases. Worcester, MA, USA/University of Massachusetts Medical School. Department of Medicine. Program in Innate Immunity. | pt_BR |
Affilliation | University of Massachusetts Medical School. Division of Infectious Diseases. Worcester, MA, USA/ University of Massachusetts Medical School. Department of Medicine. Program in Innate Immunity/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Subject | type I interferon | pt_BR |
Subject | cGAS | pt_BR |
Subject | STING | pt_BR |
Subject | TLR4 | pt_BR |
Subject | Neisseria gonorrhoeae | pt_BR |