Author | Souza, Luana Dias de | |
Author | Vendrame, Célia Maria Vieira | |
Author | Jesus, Amélia Ribeiro de | |
Author | Carvalho, Márcia Dias Teixeira | |
Author | Schriefer, Albert | |
Author | Guimarães, Luiz Henrique | |
Author | Carvalho Filho, Edgar Marcelino | |
Author | Goto, Hiro | |
Access date | 2017-04-10T18:25:55Z | |
Available date | 2017-04-10T18:25:55Z | |
Document date | 2016 | |
Citation | SOUZA, L. D. et al. Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis. Parasites & Vectors, v. 9, p. 335, 2016. | pt_BR |
ISSN | 1756-3305 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18349 | |
Description | Carvalho, E. M. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (research fellowship 2008/2209-6 to LDS), Fundação de Amparo à Pesquisa do Estado da Bahia (research fellowship 1780–2007 to CMVV), Conselho Nacional de Pesquisa (fellowship to EMC, ARJ and HG, and grant 484499/2006) and the
Soroepidemiology and Immunobiology Laboratory LIM/38 (HC-FMUSP). | pt_BR |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Leishmaniose tegumentar | pt_BR |
Subject in Portuguese | THP-1 | pt_BR |
Subject in Portuguese | Nível de IGF-I | pt_BR |
Title | Insulin-like growth factor-I serum levels and their biological effects on Leishmania isolates from different clinical forms of American tegumentary leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13071-016-1619-x | |
Abstract | American tegumentary leishmaniasis (ATL) in Brazil is mostly caused by Leishmania (Viannia) braziliensis, with known forms of the disease being cutaneous (CL), mucosal (ML) and disseminated (DL) leishmaniasis. The development of the lesion in ATL is related both to the persistence of the Leishmania in the skin and to the parasite-triggered immune and inflammatory responses that ensue lesions. In this context one factor with expected role in the pathogenesis is insulin-like growth factor (IGF)-I with known effects on parasite growth and healing and inflammatory processes. In the present study, we addressed the effect of IGF-I on intracellular amastigote isolates from CL, ML and DL patients within human macrophage and we evaluated the IGF-I and IGF-binding protein-3 (IGFBP3) serum levels in patients presenting different clinical forms and controls from the endemic area. Methods: We evaluated biological variability in the responses of intracellular amastigotes of Leishmania isolates derived
from CL, ML, and DL patients from an area for ATL in response to IGF-I. Intracellular amastigote growth was evaluated using
the human macrophage cell line THP-1. Arginase activity in infected cells was evaluated quantifying the generated urea
concentration. Serum samples from patients and controls were assayed using chemiluminescent immunometric assay to
determine IGF-I and IGFBP3 levels.
Results: We observed an increase in intracellular parasitism upon IGF-I stimulus in 62.5 % of isolates from CL, in 85.7 %
from ML and only 42.8 % from DL cases. In DL, the basal arginase activity was lower than that of CL. We then evaluated
the IGF-I and IGFBP3 serum levels in patients, and we observed significantly lower levels in ML and DL than in CL and
control samples.
Conclusions: The data suggest that IGF-I is modulated distinctly in different clinical forms of tegumentary leishmaniasis.
IGF-I seemingly exerts effect on parasite growth likely contributing to its persistence in the skin in earlier phase. In addition
the decreased IGF-I serum levels may affect the modulation of inflammation and lesion healing in chronic phase. In view of
potential role of IGF-I in the pathogenesis of ATL we can speculate on therapeutic procedures taking into account the local
IGF-I level. | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Sergipe. Laboratório de Biologia Molecular. Departamento de Medicina Interna e Patologia. Aracaju, SE, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil / | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, Brasil | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Tegumentary leishmaniasis | pt_BR |
Subject | THP-1 | pt_BR |
Subject | IGF-I level | pt_BR |