Author | Gomes, Regis Bernardo Brandim | |
Author | Cavalcanti, Katrine Bezerra | |
Author | Teixeira, Clarissa | |
Author | Carvalho, Augusto Marcelino Pedreira de | |
Author | Mattos, Paulo Sérgio de Morais da Silveira | |
Author | Cristal, Juqueline Rocha | |
Author | Muniz, Aline C | |
Author | Miranda, José Carlos | |
Author | Oliveira, Camila Indiani de | |
Author | Barral, Aldina Maria Prado | |
Access date | 2017-03-23T17:10:59Z | |
Available date | 2017-03-23T17:10:59Z | |
Document date | 2016 | |
Citation | GOMES, R. B. B. et al. Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005078, 2016. | pt_BR |
ISSN | 1935-2727 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18145 | |
Sponsorship | Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq - 400695/2012-0) and Fundação de Amparo à Pesquisa da Bahia (FAPESB INT 0003/2013), Brazil. | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Saliva | pt_BR |
Subject in Portuguese | Moscas | pt_BR |
Subject in Portuguese | Lutzomyia | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | Proteinas salivares | pt_BR |
Title | Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0005078 | |
Abstract | Previous works showed that immunization with saliva from Lutzomyia intermedia, a vector of Leishmania braziliensis, does not protect against experimental infection. However, L. braziliensis is also transmitted by Lutzomyia whitmani, a sand fly species closely related to Lu. intermedia. Herein we describe the immune response following immunization with Lu. whitmani saliva and the outcome of this response after L. braziliensis infection. Methods and findings
BALB/c mice immunized with Lu. whitmani saliva developed robust humoral and cellular
immune responses, the latter characterized by an intense cellular infiltrate and production
of IFN-γ and IL-10, by both CD4+ and CD8+ cells. Mice immunized as above and challenged
with L. braziliensis plus Lu. whitmani saliva displayed significantly smaller lesions
and parasite load at the challenge site. This protection was associated with a higher
(p<0.05) IFN-γ production in response to SLA stimulation. Long-term persisting immunity
was also detected in mice immunized with Lu. whitmani saliva. Furthermore, individuals
residing in an endemic area for cutaneous leishmaniasis (CL) presented antibody
responses to Lu. whitmani saliva. However CL patients, with active lesions, displayed a
lower humoral response to Lu. whitmani saliva compared to individuals with subclinical
Leishmania infection.
Conclusion
Pre-exposure to Lu. whitmani saliva induces protection against L. braziliensis in a murine
model. We also show that Lu. whitmani salivary proteins are immunogenic in naturally exposed individuals. Our results reinforce the importance of investigating the immunomodulatory
effect of saliva from different species of closely related sand flies. | pt_BR |
Affilliation | Fundação Oswaldo Cruz (FIOCRUZ). Escritório Técnico Regional. Teresina, PI, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz (FIOCRUZ). Escritório Técnico Regional. Teresina, PI, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Medicine. Salvador, BA, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / INCT. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / INCT. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Subject | Saliva | pt_BR |
Subject | Sand | pt_BR |
Subject | Lutzomyia | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Infection | pt_BR |
Subject | Salivary proteins | pt_BR |