Author | Ramos, Pablo Ivan Pereira | |
Author | Custódio, Márlon Grégori Flores | |
Author | Quispe Saji, Guadalupe Del Rosario | |
Author | Cardoso, Thiago | |
Author | Silva, Gisele Lucchetti da | |
Author | Braun, Graziela | |
Author | Martins, Willames Marco Brasileiro da Silva | |
Author | Girardello, Raquel | |
Author | Vasconcelos, Ana Tereza Ribeiro de | |
Author | Fernández, Elmer | |
Author | Gales, Ana Cristina | |
Author | Nicolás, Marisa Fabiana | |
Access date | 2017-03-20T18:38:20Z | |
Available date | 2017-03-20T18:38:20Z | |
Document date | 2016 | |
Citation | RAMOS, P. I. P. et al. The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, v. 17, Suppl 8, p. 737, 2016. | pt_BR |
ISSN | 1471-2164 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18085 | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) supported this work through grants awarded to MFN (process no. E-26/110.315/2014) and ACG (process no. 2010/12891-9), respectively. The publication charges for this article were funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (process no. 23038.010041/2013-13) | pt_BR |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Resistência aos antibióticos | pt_BR |
Subject in Portuguese | Klebsiella pneumoniae | pt_BR |
Subject in Portuguese | Patógeno | pt_BR |
Subject in Portuguese | Polimixina B | pt_BR |
Subject in Portuguese | RNA-seq | pt_BR |
Title | The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12864-016-3070-y | |
Abstract | The emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the
original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH),
magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control
condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond
to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the
participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K.
pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which
correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K.
pneumoniae that could relate to surviving against the action of this antibiotic.
Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions
and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond
damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets. | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Affilliation | Universidad Católica de Córdoba, CONICET. Facultad de Ingeniería. Córdoba, Argentina | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, Brasil | pt_BR |
Affilliation | Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil | pt_BR |
Subject | Antibiotic resistance | pt_BR |
Subject | Klebsiella pneumoniae, | pt_BR |
Subject | Pathogen | pt_BR |
Subject | Polymyxin B | pt_BR |
Subject | RNA-seq | pt_BR |
Subject | Transcriptomics | pt_BR |