Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18081
Title: Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
Authors: Ferreira, Paulo Michel Pinheiro
Bezerra, Daniel Pereira
Silva, Jurandy do Nascimento
Costa, Marcília Pinheiro da
Ferreira, José Roberto de Oliveira
Alencar, Nylane Maria Nunes
Figueiredo, Ingrid Samantha Tavares de
Cavalheiro, Alberto José
Machado, Camila Maria Longo
Chammas, Roger
Alves, Ana Paula Negreiros Nunes
Moraes, Manoel Odorico de
Pessoa, Claudia
Affilliation: Federal University of Piauí. Laboratory in Experimental Cancerology. Department of Biophysics and Physiology.Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, Brazil
Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Department of Pharmacy. Teresina, PI, Brazil
Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
State University of São Paulo. Chemistry Institute. Araraquara, SP, Brazil
State University of SãoPaulo. Center for Medical Nuclear. Radioisotopes Research Laboratory. São Paulo, SP, Brazil
State University of Paulo. São Faculty of Medicine. Department of Radiology. São Paulo, SP, Brazil
Federal University of Ceará. Department of Clinical Odontology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil / Oswaldo Cruz Foundation. Fortaleza, CE, Brazil
Abstract: Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim ofthestudy: Toassessthe in vivoandexvivo antitumor actionofafractionwithcasearins(FC)andits main component-CasearinX-isolatedfrom C. sylvestris leaves. Materials andmethods: Firstly,Sarcoma180bearingSwissmiceweretreatedwithFCandCasXfor 7 days.Secondly,BALB/cnudeanimalsreceivedhollow fibers withcoloncarcinoma(HCT-116)orglio- blastoma (SF-295)cellsandweretreatedwithFCfor4days.On5thday,proliferationwasdeterminedby MTT assay. Results: FC 10and25mg/kg/dayi.p.and50mg/kg/dayoralandCasX25mg/kg/dayi.p.and50mg/kg/ day oralrevealedtumorgrowthinhibitionratesof35.8,86.2,53.7,90.0and65.5%andsuchtumors demonstratedraremitosesandcoagulationnecrosisareas.Similarly,FCreducedmultiplyingofHCT-116 and SF-295cellswhenevaluatedbytheHollowFiberAssay(2.5and5mg/kg/dayi.p.and25and50mg/ kg/day oral),withcellgrowthinhibitionratesrangingfrom33.3to67.4%(po0.05). Flowcytometry experimentsrevealedthatFCreducedmembraneintegrityandinducedDNAfragmentationandmi- tochondrial depolarization(po0.05). Conclusions: FC andCasXwereefficient antitumorsubstancesagainstmurineandhumancancercells and causedreversiblemorphologicalchangesinliver,kidneysandspleens,emphasizingclerodanedi- terpenes asanemergingclassofanticancermolecules.
Keywords: Casearin X
Hollow fiber assay
Sarcoma 180
Antineoplastic
Leukogram
keywords: Casearin X
Teste de fibra oca
Sarcoma 180
Leucograma
Antineoplásico
Issue Date: 2016
Publisher: Elsevier
Citation: FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016.
DOI: 10.1016/j.jep.2016.04.011
ISSN: 0378-8741
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

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