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Título: In vivo antitumor effect, induction of apoptosis and safety of Remirea maritima Aubl. (Cyperaceae) extracts
Autor : Dória, Grace Anne Azevedo
Menezes, Paula dos Passos
Lima, Bruno S
Vasconcelos, Bruno S
Silva, Francilene Amaral da
Henriques, Raíssa Melo
Melo, Marcélia Garcez Dória de
Alves, Ângela V F
Moraes, Manoel O
Pessoa, Cláudia Ó
Carvalho, Adriana A
Prata, Ana Paula do Nascimento
Albuquerque Junior, Ricardo Luiz Cavalcanti de
Verde, Isabel Bezerra Lima
Quintans Júnior, Lucindo José
Bezerra, Daniel Pereira
Nogueira, Paulo C L
Araujo, Adriano A S
Afiliación: Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Pharmacy. São Cristóvão, SE, Brazil
Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil
Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Biology. São Cristóvão, SE, Brazil
Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil
Tiradentes University. Institute of Technology and Research. Aracaju, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Sergipe. Department of Chemistry. São Cristóvão, SE, Brazil
Resumen: Remirea maritima has been widely used in the treatment of diarrhea, kidney disease, and high fever and for therapeutic purposes, such as an analgesic and anti-inflammatory. However, few scientific research studies on its medicinal properties have been reported. Purpose: The present study aimed to investigate the anticancer potential of aqueous extract (AE), 40% hydroalcoholic extracts (40HA) and 70% (70HA) from R. maritima in experimental models and to identify its phytochemical compounds. Methods: The chemical composition of AE, 40HA and 70HA was assessed by HPLC-DAD and ESI-IT-MS/MS . In vitro activity was determined on cultured tumor cell, NCI-H385N (Broncho-alveolar carcinoma), OVCAR- 8 (Ovarian carcinoma) and PC-3 M (prostate carcinoma) by the MTT assay, and the in vivo antitumor activity was assessed in Sarcoma 180-bearing mice. Toxicological parameters were also evaluated as well as the humoral immune response. Results: Among the aqueous and hydroalcoholic extracts of R. maritima , only 40HA showed in vitro bi- ological effect potential, presenting IC 50 values of 27.08, 46.62 and > 50 μg/ml for OVCAR-8, NCI-H385M and PC-3 M cells lines, respectively. Regarding chemical composition, a mixture of isovitexin-2 - O - β-D- glucopyranoside, vitexin-2 - O - β-D-glucopyranoside, luteolin-7- O -glucuronide and 1- O -(E)-caffeoyl- β-D- glucose were identified as the major phytochemical compounds of the extracts. In the in vivo study, the tumor inhibition rates were 57.16–62.57% at doses of 25 mg/kg and 50 mg/kg, respectively, and the tu- mor morphology presented increasing numbers of apoptotic cells. Additionally, 40HA also demonstrated significantly increased of OVA-specific total Ig. Conclusions: 40HA exhibited in vitro and in vivo anticancer properties without substantial toxicity that could be associated with its immunostimulating properties.
Palabras clave en ingles: Remirea maritima
Antitumor
Sarcoma 180
Apoptosis
Toxicity
Tunel
Palabras clave en portugues: Remirea maritima
Antitumor
Sarcoma 180
Apoptose
Toxicidade
Túnel
Fecha de publicación: 2016
Editorial : Elsevier
Referencia: DÓRIA, G. A. A. et al. In vivo antitumor effect, induction of apoptosis and safety of Remirea maritima Aubl. (Cyperaceae) extracts. Phytomedicine, v. 23, p. 914–922, 2016.
DOI: 10.1016/j.phymed.2016.05.001
ISSN : 0944-7113
Derechos de autor: open access
Aparece en las colecciones: CPqGM - Artigos de Periódicos

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