Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18034
Title: Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma
Authors: Almeida, Tarcísio Vila Verde Santana de
Fernandes, Jamille Souza
Lopes, Diego Mota
Andrade, Lorena Santana
Oliveira, Sérgio Costa
Carvalho, Edgar Marcelino
Araujo, Maria Ilma
Cruz, Álvaro A
Cardoso, Luciana Santos
Affilliation: Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Universidade Federal de Minas Gerias. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / UFBA. ProAR—Núcleo de Excelência em Asma. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / UFBA. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
Abstract: Asthma is a chronic disease characterized by airway inflammation, obstruction and hyperresponsiveness. Severe asthma affects a small proportion of subjects but results in most of the morbidity, costs and mortality associated with the disease. Studies have suggested that Schistosoma mansoni infection reduces the severity of asthma and prevent atopy. Objective: We evaluated the ability of S. mansoni antigens, Sm29 and Sm29TSP-2 to modulate lymphocyteactivation status in response to the allergen of the mite Dermatophagoides pteronyssinus (Der p1) in cellcultures of individuals with asthma.Methods: Thirty four patients were enrolled in this study: seventeen patients with severe asthma (SAgroup), seventeen patients with mild asthma (MA group) and six controls with no asthma. Peripheralblood mononuclear cells (PBMC) were obtained and stimulated with Sm29 and Sm29TSP-2 in the pres-ence or absence of Der p1. The expression of surface markers and cytokines on lymphocytes was evaluatedby flow cytometry and the levels of IL-10 in the culture supernatant were determined by ELISA.Results: The addition of Sm29 and Sm29TSP-2 antigens to PBMC cultures from both groups of subjectswith asthma stimulated with Der p1 reduced the frequency of CD4+CD25lowcells whereas and increasedfrequency of CD4+CD25highpopulation was observed compared to unstimulated cultures. Moreover, cul-tures stimulated with Sm29TSP-2 showed a reduction in the frequency of T cells expressing CD69, IFN- ,TNF and TGF- in the MA group and an increase in the frequency of CD4+TSLPR+T cells in the SA group.The addition of Sm29 to the cultures reduced the frequency of CD4+CD69+and CD4+IL-5+T cells in all asth-matic groups, and reduced the frequency of CD4+T cells expressing IL-13 in the MA group. The culturesstimulated with Sm29 and Sm29TSP-2 showed an increase in the level of IL-10 in the supernatants.Conclusion: These results suggest that the addition of Sm29 and Sm29TSP-2 to the cells cultures fromsubjects with asthma reduced cell activation markers and altered the cytokine production pattern in away that can potentialy control the inflammatory response associated with asthma
Keywords: Asthma
Schistosoma mansoni antigens
Lymphocytes
Sm29
Sm29TSP-2a
keywords: Asma
Antígenos de Schistosoma mansoni
Linfócitos
Sm29
Sm29TSP-2a
Issue Date: 2017
Publisher: Elsevier
Citation: ALMEIDA, T. V. V. S. et al. Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma. Acta Tropica, v. 166, p. 268–279, 2017.
DOI: 10.1016/j.actatropica.2016.12.002
ISSN: 0001-706X
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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