Author | Lima, Luciane Mota | |
Author | Cardoso, Luciana Santos | |
Author | Santos, Silvane Braga | |
Author | Oliveira, Ricardo Riccio | |
Author | Oliveira, Sérgio Costa | |
Author | Góes, Alfredo Miranda | |
Author | Loukas, Alex | |
Author | Araujo, Maria Ilma | |
Access date | 2017-03-06T19:04:37Z | |
Available date | 2017-03-06T19:04:37Z | |
Document date | 2017 | |
Citation | LIMA, L. M. et al. Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals. Acta Tropica, v. 167, p. 157–162, 2017. | pt_BR |
ISSN | 0001-706X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/17949 | |
Sponsorship | FundingCNPQ (Universal 479417/2008 3), NIH (R01AI079238A). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | HTLV-1 | pt_BR |
Subject in Portuguese | Schistosoma | pt_BR |
Subject in Portuguese | Proteínas | pt_BR |
Subject in Portuguese | CXCL9 | pt_BR |
Subject in Portuguese | CXCL10 | pt_BR |
Title | Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.actatropica.2016.12.030 | |
Abstract | HTLV-1 is the causal agent of Adult T cell Leukemia/lymphoma (ATLL) and HTLV-1-associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). The immune response to HTLV-1-infection is polarized to the Th1-type, and the presence of CXCL9/CXCL10 chemokines may lead to an increase in the recruitment of pro-inflammatory molecules in spinal cord tissue, contributing to the damage observed in the development of HAM/TSP. It has been observed that in chronic helminth-infections, such as schistosomiasis, there is a deviation toward the Th2/regulatory immune response. Objective: To evaluate the ability of Schistosoma spp. proteins to decrease the in vitro CXCL9 and CXCL10
production by PBMC of HTLV-1-infected individuals.
Methods: The Schistosoma proteins rSm29, rSh-TSP-2 and PIII were added to PBMC cultures of HTLV-1-
infected individuals and the levels of chemokines in the supernatants were measured using a sandwich
ELISA method.
Results: The addition of rSm29 to the cultures resulted in decreased production of CXCL9 in all the analyzed
individuals and HAM/TSP group (18167
±
9727 pg/mL, p = 0.044; 20237
±
6023 pg/mL, p = 0.028,
respectively) compared to the levels in unstimulated cultures (19745
±
9729 pg/mL; 25078
±
2392 pg/mL,
respectively). The addition of rSh-TSP-2 decreased the production of CXCL9 in all studied individuals and
carriers group (16136
±
9233 pg/mL, p = 0.031; 13977
±
8857 pg/mL, p = 0.026) vs unstimulated cultures
(19745
±
9729 pg/mL; 18121
±
10508 pg/mL, respectively). Addition of PIII did not alter the results. There
was no significant change in the levels of CXCL10 by the addition of the studied proteins.
Conclusion: The Schistosoma proteins used in this study were able to down modulate the production of
CXCL9, a chemokine associated with the inflammatory process in HTLV-1-infection. | pt_BR |
Affilliation | Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. INCT-DT. CNPq/MCT. Salvador, BA, Brasil | pt_BR |
Affilliation | Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Australian Institute of Tropical Health and Medicine, James Cook University, Queensland, Australia | pt_BR |
Affilliation | Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública, Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. INCT-DT. CNPq/MCT. Salvador, BA, Brasil | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | Schistosoma proteins | pt_BR |
Subject | CXCL9 | pt_BR |
Subject | CXCL10 | pt_BR |