Author | Brustolim, Daniele | |
Author | Vasconcelos, Juliana Fraga | |
Author | Freitas, Luiz Antonio Rodrigues de | |
Author | Teixeira, Mauro Martins | |
Author | Farias, Marcel T. | |
Author | Ribeiro, Yvone M. | |
Author | Tomassini, Therezinha Coelho Barbosa | |
Author | Oliveira, Geraldo Gileno de Sá | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Soares, Milena Botelho Pereira | |
Access date | 2011-03-29T15:55:49Z | |
Available date | 2011-03-29T15:55:49Z | |
Document date | 2010 | |
Citation | BRUSTOLIM, D. et al. Activity of Physalin F in a Collagen-Induced Arthritis Model. Journal of Natural Products, v.73, n.8, p.1323-1326, 2010. | pt_BR |
xmlui.metadata.dc.identifier.other | 20681573 | |
URI | https://www.arca.fiocruz.br/handle/icict/1790 | |
Language | eng | pt_BR |
Rights | open access | |
Title | Activity of Physalin F in a Collagen-Induced Arthritis Model | pt_BR |
Type | Article | pt_BR |
DOI | 10.1021/np900691w | |
Abstract | The effects of physalin F (1), a steroid derivative purified from Physalis angulata, were investigated in models of collagen-induced arthritis in DBA/1 mice and allergic airway inflammation in BALB/c mice. Oral treatment with 1 or dexamethasone caused a marked decrease in paw edema and joint inflammation when compared to vehicle-treated arthritic mice. In contrast, treatment with 1 had no effect in mice with allergic airway inflammation caused by ovalbumin immunization, whereas dexamethasone significantly reduced the number of inflammatory cells and eosinophils in the broncoalveolar lavage fluid and in lung sections of challenged mice. To further demonstrate that 1 acts through a mechanism different from that of glucocorticoids, a nuclear translocation assay was performed of the glucocorticoid receptor (GR) using COS-7 cells transfected with a plasmid encoding for a yellow fluorescent protein (YFP)-GR fusion protein. Untreated or treated cells with 1 had YFP staining mainly in the cytoplasm, whereas in dexamethasone-treated cells the YFP staining was concentrated in the nuclei. It is concluded that the mechanism of the immunosuppressive activity of physalin F is distinct from that of the glucocorticoids. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Minas Gerais, BH, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil | |
Affilliation | Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil | |
DeCS | Antiinflamatórios/PD | pt_BR |
DeCS | Modelos Animas de Doenças | pt_BR |
DeCS | Secoesteróides | pt_BR |
DeCS | Artrite Experimental | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |