Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/17854
Title: Scoring clinical signs can help diagnose canine visceral leishmaniasis in a highly endemic area in Brazil
Authors: Silva, Kleverton Ribeiro da
Mendonça, Vitor Rosa Ramos de
Silva, Kellen Matuzzy
Nascimento, Leopoldo Fabrício Marçal do
Sousa, Antonio Ferreira Mendes
Pinho, Flaviane Alves de
Barral-Netto, Manoel
Barral, Aldina Maria Prado
Cruz, Maria do Socorro Pires E
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Piauí. Departamento de Morfofisiologia Veterinária. Teresina, PI, Brasil
Universidade Federal do Piauí. Departamento de Morfofisiologia Veterinária. Teresina, PI, Brasil
Universidade Federal do Piauí. Picos, PI, Brasil
Universidade Federal do Piauí. Departamento de Morfofisiologia Veterinária. Teresina, PI, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Universidade Federal do Piauí. Departamento de Morfofisiologia Veterinária. Teresina, PI, Brasil
Abstract: Canine visceral leishmaniasis (CVL) diagnosis is still a challenge in endemic areas with limited diagnostic resources. This study proposes a score with the potential to distinguish positive CVL cases from negative ones. We studied 265 dogs that tested positive for CVL on ELISA and parasitological tests. A score ranging between 0 and 19 was recorded on the basis of clinical signs. Dogs with CVL had an overall higher positivity of the majority of clinical signs than did dogs without CVL or with ehrlichiosis. Clinical signs such as enlarged lymph nodes (83.93%), muzzle/ear lesions (55.36%), nutritional status (51.79%), bristle condition (57.14%), pale mucosal colour (48.21%), onychogryphosis (58.93%), skin lesion (39.28%), bleeding (12.50%), muzzle depigmentation (41.07%), alopecia (39.29%), blepharitis (21.43%), and keratoconjunctivitis (42.86%) were more frequent in dogs with CVL than in dogs with ehrlichiosis or without CVL. Moreover, the clinical score increased according to the positivity of all diagnostic tests (ELISA, p < 0.001; parasite culture, p = 0.0021; and smear, p = 0.0003). Onychogryphosis (long nails) [odds ratio (OR): 3.529; 95% confidence interval (CI): 1.832-6.796; p < 0.001], muzzle depigmentation (OR: 4.651; 95% CI: 2.218-9.750; p < 0.001), and keratoconjunctivitis (OR: 5.400; 95% CI: 2.549-11.441; p < 0.001) were highly associated with CVL. Interestingly, a score cut-off value ≥ 6 had an area under the curve of 0.717 (p < 0.0001), sensitivity of 60.71%, and specificity of 73.64% for CVL diagnosis. The clinical sign-based score for CVL diagnosis suggested herein can help veterinarians reliably identify dogs with CVL in endemic areas with limited diagnostic resources.
Keywords: Canine visceral leishmaniasis
Clinical score
Diagnosis
Clinical signs
keywords: Leishmaniose visceral canina
Escore clínico
Diagnóstico
Sinais clínicos
Issue Date: 2017
Publisher: Instituto Oswaldo Cruz
Citation: SILVA, K. R. et al. Scoring clinical signs can help diagnose canine visceral leishmaniasis in a highly endemic area in Brazil. Memórias do Instituto Oswaldo Cruz, v. 112, n. 1 p. 53-62, 2017.
DOI: 10.1590/0074-02760160305
ISSN: 0074-0276
Copyright: open access
Appears in Collections:CPqGM - Artigos de Periódicos

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