Author | Matos, Christiane Santos | |
Author | Reis, Jordana Grazziela Alves Coelho dos | |
Author | Rassi, Anis | |
Author | Luquetti, Alejandro Ostermayer | |
Author | Dias, João Carlos Pinto | |
Author | Santos, Silvana Maria Eloi | |
Author | Gomes, Izabelle Teixeira | |
Author | Avelar, Danielle Marquete Vitelli | |
Author | Wendling, Ana Paula Barbosa | |
Author | Rocha, Roberta Dias Rodrigues | |
Author | Carvalho, Andréa Teixeira de | |
Author | Pascoal, Vanessa Peruhype Magalhães | |
Author | Andrade, Mariléia Chaves de | |
Author | Martins Filho, Olindo Assis | |
Access date | 2017-01-19T18:05:27Z | |
Available date | 2017-01-19T18:05:27Z | |
Document date | 2011 | |
Citation | MATOS, Christiane Santos et al. Applicability of an optimized non-conventional flow cytometry method to detect anti-Trypanosoma cruzi immunoglobulin G for the serological diagnosis and cure assessment following chemotherapeutic treatment of Chagas disease. J Immunol Methods, vol. 369, p. 22-32, 2011. | pt_BR |
ISSN | 0022-1759 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16900 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Doença de chagas | pt_BR |
Title | Applicability of an optimized non-conventional flow cytometry method to detect anti-Trypanosoma cruzi immunoglobulin G for the serological diagnosis and cure assessment following chemotherapeutic treatment of Chagas disease | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jim.2011.03.007 | |
Abstract | One of the challenges on immunodiagnostic of Chagas disease in endemic areas has been the search for more practical and safe antigenic preparation that provides tests with higher sensitivity and specificity, with low cross-reactivity. A new approach using fixedTrypanosoma cruzi epimastigotes to detect IgG reactivity was investigated previously. In order to continue this investigation, this study aimed at optimizing the flow cytometry-based method to the diagnosis of Chagas disease patients after specific chemotherapy. To achieve our goal, serum samples from 93 subjects — 52 adults chronically infected byT. cruzi, and 41 uninfected controls were tested by flow cytometry. Secondly, serum samples from patients Treated Cured and Treated Uncured from Chagas disease were also tested to evaluate the potential of the method on assessing cure. After establishing the ideal serum dilution and cut off, 121 serum samples from patients with other endemic infections were tested to check cross-reactivity. The results showed that parasite staining with Evan's blue dye eliminated debris, allowing trustworthy analysis of anti-fixed epimastigote IgG reactivity. The applicability of the method to diagnose Chagas disease was confirmed by the high sensitivity (98.1%) and specificity (100%) found. This method also contributed for post-therapeutic assessment of cure, identifying 94.1% of Treated Uncured and 83.3% of Treated Cured patients. Cross-reactivity was observed in a very low number (6.7%). On the whole, these data highly recommend the use of anti-fixed T. cruzi epimastigote IgG reactivity by flow cytometry to the diagnosis and cure monitoring of Chagas disease in endemic areas. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Goiás. Faculdade de Medicina. Goiânia, GO, Brazil | pt_BR |
Affilliation | Universidade Federal de Goiás. Faculdade de Medicina. Goiânia, GO, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de propedêutica Complementar. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil /Universidades Estadual de Montes Claros. Departamento de Fisiopatologia. Montes Claros, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | Epimastigote | pt_BR |
Subject | Diagnosis | pt_BR |
Subject | Cure monitoring | pt_BR |
Embargo date | 2030-01-01 | |