Author | Oliveira, Diêgo Madureira de | |
Author | Lima, Rute Maria Ferreira | |
Author | Clarencio, Jorge | |
Author | Velozo, Eudes da Silva | |
Author | Amorim, Ilza Alves de | |
Author | Mota, Tales Henrique Andrade da | |
Author | Costa, Silvia Lima | |
Author | Silva, Fábio Pittella | |
Author | El-Bachá, Ramon Dos Santos | |
Access date | 2016-12-14T19:14:36Z | |
Available date | 2016-12-14T19:14:36Z | |
Document date | 2016 | |
Citation | OLIVEIRA, D. M. et al. The classical photoactivated drug 8-methoxypsoralen and related compounds are effective without UV light irradiation against glioma cells. Neurochemistry International, v. 99, p. 33-41, 2016 | pt_BR |
ISSN | 0197-0186 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16531 | |
Sponsorship | CNPq | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Glioma | pt_BR |
Subject in Portuguese | 8-metoxipsoraleno | pt_BR |
Subject in Portuguese | Psoraleno | pt_BR |
Subject in Portuguese | Coumarinas | pt_BR |
Title | The classical photoactivated drug 8-methoxypsoralen and related compounds are effective without UV light irradiation against glioma cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.neuint.2016.06.004 | |
Abstract | Currently, there is no effective therapy for high grade gliomas. 8-Methoxypsoralen (8-MOP) is a compound used in the treatment of skin diseases combined with UV light irradiation. In this work, rat glioma C6 cells, normal astrocytes and human glioblastoma GL-15 cells comprised an in vitro model to evaluate the antitumor activity of 8-MOP. We found that 8-MOP promoted a time- and concentration-dependent reduction of cell viability in tumor, but not in normal cells. This effect was more evident in log-phase growing culture, indicating antiproliferative activity, which was confirmed by colony formation assay. Long-term effect of 8-MOP at low concentration was also attested. The concentrations used in the tests (0.02-0.4 mM) were lower than plasmatic concentration found in patients. Despite the treatment leads to considerable morphological changes and apoptosis when used at high concentrations, 8-MOP did not promote cell cycle arrest, change in migration pattern neither necrosis. In addition, we evaluated the effect of 8-MOP in MDA-MB-231, CT-26 and SCC-3 cell lines, derived from other kind of primary tumors, and found that CT-26 cells did not respond to 8-MOP treatment, indicating that this compound does not act through a generic mechanism. Coumarin derivatives structurally related to 8-MOP were screened for its antitumor potential and presented different patterns of biological activity, and then it was possible to suggest the relevance of 8-MOP molecular structure for antiproliferative action. Therefore, 8-MOP, a drug with an outstanding record of safety, and related coumarins are good prototypes for development of a new class of anti-glioma drugs. | pt_BR |
Affilliation | University of Brasilia. Department of Biological Basis of Health Sciences. Brasília, DF, Brasil / University of Brasilia. Laboratory of Molecular Pathology of Cancer. Brasília, DF, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Institute of Health Sciences. Laboratory of Neurochemistry and Cell Biology. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / School of Medicine. Public Health of Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Faculty of Pharmacy. LAPEMM. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Faculty of Pharmacy. LAPEMM. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Brasilia. School of Pharmacy. Ceilândia, DF, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Institute of Health Sciences. Laboratory of Neurochemistry and Cell Biology. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Brasilia. Laboratory of Molecular Pathology of Cancer. Brasília, DF, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Institute of Health Sciences. Laboratory of Neurochemistry and Cell Biology. Salvador, BA, Brasil | pt_BR |
Subject | Glioma | pt_BR |
Subject | 8-methoxypsoralen | pt_BR |
Subject | Psoralen | pt_BR |
Subject | Coumarins | pt_BR |