Author | Rosa, Juan | |
Author | Pereira, Daniela Pita | |
Author | Brazil, Reginaldo Peçanha | |
Author | Andrade Filho, José Dilermando | |
Author | Salomón, Oscar | |
Author | Szelag, Enrique | |
Access date | 2016-10-13T15:23:45Z | |
Available date | 2016-10-13T15:23:45Z | |
Document date | 2012 | |
Citation | ROSA, Juan; et al. Natural infection of cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco, Argentina. Acta Tropica, v.123, n.2, p.128-131, Aug. 2012. | pt_BR |
ISSN | 0001-706X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16217 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Hibridização | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Subject in Portuguese | Chaco seco | pt_BR |
Subject in Portuguese | Leishmaniose Tegumentar Americana | pt_BR |
Title | Natural infection of cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco, Argentina | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.actatropica.2012.04.008 | |
Abstract | In Argentina, American Cutaneous Leishmaniasis (ACL) extends up to 29°S in the phytogeographic regions of the Yungas (west), Chaco (center) and Paranaense (east). Since the Phlebotominae vectors of this disease in the western Chaco (dry Chaco) are unknown, in the present work, we studied the natural infection in Phlebotominae by PCR-ERFLP and Dot blot in order to incriminate these organisms as potential vectors. Captures with CDC-type traps were performed monthly in the domicile, the peridomicile and the forest in the Municipio Misión Nueva Pompeya, Chaco, Argentina, in two sites with human cases of ACL: Los Pozos (24°54'S, 61°22'W) and Fortín Arenales (24°58'S, 61°21'W), from November 2006 to December 2007. A total of 1702 Phlebotominae were captured: Mygonemyia migonei (83.8%), cortelezzii complex (11.1%), Mycropigomyia peresi (3.3%), Mycropygomy quinquefer (1.2%), Pintomyia torresi (0.2%) and Nyssomyia neivai (0.2%). Although no significant differences were found in species diversity, there were significant differences in abundance between both sites studied. A total of 80 phlebotomine females were analyzed: 50 of the cortelezzii complex and 30 My. migonei. No intestinal flagellates were observed by light microscopy. Two pools of 10 individuals of the cortelezzii complex of the peridomicile and forest of Fortín Arenales were reactive by PCR and Dot blot for Leishmania (Viannia) braziliensis. In Argentina, Evandromyia cortelezzii has been incriminated as a likely vector of ACL because of its abundance in areas of sporadic outbreaks. In the present work, Ev. cortelezzii females were found naturally infected, thus reinforcing the hypothesis that the members of the cortelezzii complex act as vectors of the disease. | pt_BR |
Affilliation | Universidad Nacional del Nordeste. Instituto de Medicina Regional. Área Entomologia. Resistencia, Chaco, Argentina. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Instituto Nacional de Medicina Tropical, Neuquen y Jujuy. Puerto Iguazu, Misiones, Argentina. | pt_BR |
Affilliation | Universidad Nacional del Nordeste. Instituto de Medicina Regional. Área Entomologia. Resistencia, Chaco, Argentina. | pt_BR |
Subject | Dry Chaco | pt_BR |
Subject | ACL vector | pt_BR |
Subject | Multiplex PCR assay | pt_BR |
Subject | Dot blot analysis | pt_BR |
Subject | Hybridization | pt_BR |
Subject | American Cutaneous Leishmaniasis | pt_BR |
e-ISSN | 1873-6254 | |
Embargo date | 2030-01-01 | |