Author | Koishi, Andrea Cristine | |
Author | Aoki, Mateus Nóbrega | |
Author | Jorge, Taissa Ricciardi | |
Author | Suzukawa, Andréia Akemi | |
Author | Zanluca, Camila | |
Author | Levis, Silvana | |
Author | Santos, Claudia Nunes Duarte dos | |
Access date | 2016-10-07T18:45:18Z | |
Available date | 2016-10-07T18:45:18Z | |
Document date | 2016 | |
Citation | KOISHI, A., C., et al. Development and validation of a point-of-care test for detecting hantavirus antibodies in human and rodent samples. Diagn Microbiol Infect Dis., v. 85, n. 3, p. 323-327, July 2016. | pt_BR |
ISSN | 0732-8893 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16154 | |
Sponsorship | CNPq, Fundação Araucária, CAPES, PROBIO | pt_BR |
Language | eng | pt_BR |
Publisher | Mark Holodniy | pt_BR |
Rights | open access | pt_BR |
Title | Development and validation of a point-of-care test for detecting hantavirus antibodies in human and rodent samples | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.diagmicrobio.2016.04.010 | pt_BR |
Abstract | Hantaviruses are etiologic agents of a zoonotic disease transmitted mainly from wild rodents to humans, causing Hemorrhagic Fever with Renal Syndrome in Eurasia and the Hantavirus Cardiopulmonary Syndrome in the Americas (HCPS), reaching a lethality rate of 40% in Brazil. Hantavirus diagnostic and seroprevalence are often based on the presence of IgM and IgG antibodies against the virus. Here we propose a rapid test assay able to identify hantavirus antibodies with sensibility and specificity similar to ELISA assays. We analyzed five groups of samples, including healthy human population and small mammals of endemic areas, suspected cases of HCPS, patients with non-related infections and a serum panel from a different geographical region. The test presented good rates of sensibility (87-100%) and specificity (97-100%) for all groups, being a promising tool suitable for both rodent and human hantavirus epidemiological surveys. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Affilliation | Instituto Nacional de Enfermedades Virales Humanas Dr. J. I. Maiztegui, Pergamino, Argentina. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil. | pt_BR |
Subject | Hantavirus | pt_BR |
Subject | Hantavirus cardiopulmonary syndrome (HCPS) | pt_BR |
Subject | Rapid test | pt_BR |
Subject | Rodents | pt_BR |
Subject | Human seroprevalence | pt_BR |